Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5066760..5067301 Replicon chromosome
Accession NZ_CP022086
Organism Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag CEQ26_RS27405 Protein ID WP_000615974.1
Coordinates 5066760..5067038 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CEQ26_RS27410 Protein ID WP_000729704.1
Coordinates 5067041..5067301 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ26_RS27390 5064342..5064920 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CEQ26_RS27395 5065126..5065893 + 768 WP_000333380.1 class II glutamine amidotransferase -
CEQ26_RS27400 5065864..5066604 - 741 WP_001225679.1 murein L,D-transpeptidase -
CEQ26_RS27405 5066760..5067038 - 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CEQ26_RS27410 5067041..5067301 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CEQ26_RS27415 5067487..5068260 + 774 WP_000543895.1 C40 family peptidase -
CEQ26_RS27420 5068317..5068673 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CEQ26_RS27425 5068666..5068944 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
CEQ26_RS27430 5069049..5070761 - 1713 Protein_4834 flagellar biosynthesis protein FlhA -
CEQ26_RS27435 5070733..5071518 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T78394 WP_000615974.1 NZ_CP022086:c5067038-5066760 [Escherichia coli O104:H4]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T78394 NZ_CP022086:c5067038-5066760 [Escherichia coli O104:H4]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT78394 WP_000729704.1 NZ_CP022086:c5067301-5067041 [Escherichia coli O104:H4]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT78394 NZ_CP022086:c5067301-5067041 [Escherichia coli O104:H4]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References