Detailed information of TA system TA078390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4165839..4166441
• Replicon: chromosome
• Accession: NZ_CP022086
• Organism: Escherichia coli O104:H4 strain FDAARGOS_348

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CEQ26_RS22815
• Protein ID: WP_000897305.1
• Coordinates: 4165839..4166150 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEQ26_RS22820
• Protein ID: WP_000356397.1
• Coordinates: 4166151..4166441 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ26_RS22790	4161753..4162352	+	600	WP_001295269.1	glucose-1-phosphatase	-
CEQ26_RS22795	4162346..4163218	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CEQ26_RS22800	4163215..4163652	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CEQ26_RS22805	4163697..4164638	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CEQ26_RS22810	4164702..4165610	-	909	WP_001387201.1	alpha/beta hydrolase	-
CEQ26_RS22815	4165839..4166150	+	312	WP_000897305.1	hypothetical protein	Toxin
CEQ26_RS22820	4166151..4166441	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CEQ26_RS22830	4167027..4167245	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CEQ26_RS22835	4167465..4167707	+	243	WP_001087409.1	hypothetical protein	-
CEQ26_RS22840	4168037..4168966	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CEQ26_RS22845	4168963..4169598	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CEQ26_RS22850	4169595..4170497	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T78390 WP_000897305.1 NZ_CP022086:4165839-4166150 [Escherichia coli O104:H4]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T78390 NZ_CP022086:4165839-4166150 [Escherichia coli O104:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT78390 WP_000356397.1 NZ_CP022086:4166151-4166441 [Escherichia coli O104:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT78390 NZ_CP022086:4166151-4166441 [Escherichia coli O104:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7