Detailed information of TA system TA078357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4588191..4588925
• Replicon: chromosome
• Accession: NZ_CP022073
• Organism: Citrobacter koseri strain FDAARGOS_287

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CEP66_RS22650
• Protein ID: WP_058668984.1
• Coordinates: 4588620..4588925 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A8AI81
• Locus tag: CEP66_RS22645
• Protein ID: WP_012132926.1
• Coordinates: 4588191..4588532 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP66_RS22620	4583676..4584299	-	624	WP_060816116.1	thioredoxin domain-containing protein	-
CEP66_RS22625	4584296..4586182	-	1887	WP_060816117.1	thioredoxin family protein	-
CEP66_RS22630	4586231..4586596	-	366	WP_047457658.1	hypothetical protein	-
CEP66_RS22635	4586826..4587746	+	921	WP_049009332.1	curved DNA-binding protein	-
CEP66_RS22640	4587746..4588051	+	306	WP_047457663.1	chaperone modulator CbpM	-
CEP66_RS22645	4588191..4588532	-	342	WP_012132926.1	HigA family addiction module antidote protein	Antitoxin
CEP66_RS22650	4588620..4588925	-	306	WP_058668984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEP66_RS22655	4589154..4590257	+	1104	WP_058668983.1	4Fe-4S binding protein	-
CEP66_RS22660	4590291..4591067	-	777	WP_012132930.1	ABC transporter ATP-binding protein	-
CEP66_RS22665	4591067..4591837	-	771	WP_058668982.1	ABC transporter permease	-
CEP66_RS22670	4591834..4592841	-	1008	WP_049009338.1	ABC transporter substrate-binding protein	-
CEP66_RS22675	4592870..4593589	-	720	WP_058668981.1	aspartate/glutamate racemase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11958.68 Da
• Isoelectric Point: 8.3565

>T78357 WP_058668984.1 NZ_CP022073:c4588925-4588620 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKVPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T78357 NZ_CP022073:c4588925-4588620 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAGTTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.91 Da
• Isoelectric Point: 5.1864

>AT78357 WP_012132926.1 NZ_CP022073:c4588532-4588191 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Nucleotide

• Length: 342 bp

>AT78357 NZ_CP022073:c4588532-4588191 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGACCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTACAGACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A8AI81