Detailed information of TA system TA078292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4978568..4978793
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: CEP72_RS26475
• Protein ID: WP_000813258.1
• Coordinates: 4978638..4978793 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4978568..4978626 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS26425	4973633..4973875	+	243	WP_000747948.1	hypothetical protein	-
CEP72_RS26430	4973859..4974284	+	426	WP_000693878.1	Rha family transcriptional regulator	-
CEP72_RS31045	4974353..4975396	+	1044	WP_001262402.1	hypothetical protein	-
CEP72_RS31050	4975308..4975850	+	543	WP_072130322.1	replication protein	-
CEP72_RS26445	4975884..4976600	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
CEP72_RS26450	4976633..4976914	+	282	WP_000603384.1	DNA-binding protein	-
CEP72_RS26455	4976911..4977138	+	228	WP_000699809.1	hypothetical protein	-
CEP72_RS26460	4977131..4977442	+	312	WP_001289673.1	hypothetical protein	-
CEP72_RS26465	4977570..4977788	+	219	WP_000683609.1	DUF4014 family protein	-
CEP72_RS26470	4977790..4978347	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	4978568..4978626	-	59	-	-	Antitoxin
CEP72_RS26475	4978638..4978793	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
CEP72_RS26480	4978913..4979257	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
CEP72_RS26485	4979379..4979651	+	273	WP_000191872.1	hypothetical protein	-
CEP72_RS26490	4979653..4980702	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
CEP72_RS26495	4980715..4981020	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
CEP72_RS26500	4981083..4981637	+	555	WP_000640035.1	DUF1133 family protein	-
CEP72_RS26505	4981862..4982059	+	198	WP_000917763.1	hypothetical protein	-
CEP72_RS26510	4982195..4982908	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
CEP72_RS26525	4983359..4983790	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4966741..5020538	53797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T78292 WP_000813258.1 NZ_CP022050:4978638-4978793 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T78292 NZ_CP022050:4978638-4978793 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT78292 NZ_CP022050:c4978626-4978568 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin