Detailed information of TA system TA078290

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4839275..4839500
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CEP72_RS25685
• Protein ID: WP_000813254.1
• Coordinates: 4839345..4839500 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4839275..4839333 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS25640	4834426..4834833	-	408	WP_001003379.1	helix-turn-helix domain-containing protein	-
CEP72_RS25645	4834911..4835138	+	228	WP_000476986.1	transcriptional regulator	-
CEP72_RS25650	4835122..4835673	+	552	WP_000705378.1	hypothetical protein	-
CEP72_RS31000	4835645..4836685	+	1041	WP_000020565.1	DNA-binding protein	-
CEP72_RS31005	4836597..4837139	+	543	WP_157837342.1	replication protein	-
CEP72_RS25660	4837174..4837932	+	759	WP_000537579.1	DUF1627 domain-containing protein	-
CEP72_RS25665	4837992..4838177	+	186	WP_000215514.1	hypothetical protein	-
CEP72_RS25675	4838525..4839073	+	549	WP_000211435.1	ORF6N domain-containing protein	-
-	4839275..4839333	-	59	-	-	Antitoxin
CEP72_RS25685	4839345..4839500	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CEP72_RS25690	4839603..4839920	-	318	WP_000042395.1	DNA-binding transcriptional regulator	-
CEP72_RS25695	4839913..4840284	-	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CEP72_RS25700	4840508..4840735	+	228	WP_001452497.1	hypothetical protein	-
CEP72_RS25705	4840789..4841058	+	270	WP_024177817.1	hypothetical protein	-
CEP72_RS25710	4841060..4842109	+	1050	WP_088592046.1	DUF968 domain-containing protein	-
CEP72_RS25715	4842122..4842496	+	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
CEP72_RS25720	4842493..4843314	+	822	WP_000762902.1	antitermination protein	-
CEP72_RS25725	4843541..4843738	+	198	WP_000917735.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / nleH2 / nleF / espM1 / ospG	4765563..4875390	109827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T78290 WP_000813254.1 NZ_CP022050:4839345-4839500 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T78290 NZ_CP022050:4839345-4839500 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATTGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT78290 NZ_CP022050:c4839333-4839275 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin