Detailed information of TA system TA078268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1383777..1384316
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: CEP72_RS07455
• Protein ID: WP_001303998.1
• Coordinates: 1384056..1384316 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: CEP72_RS07450
• Protein ID: WP_000729705.1
• Coordinates: 1383777..1384037 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS07430	1379531..1380316	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CEP72_RS07435	1380288..1382000	+	1713	Protein_1335	flagellar biosynthesis protein FlhA	-
CEP72_RS07440	1382145..1382642	-	498	Protein_1336	REP-associated tyrosine transposase RayT	-
CEP72_RS07445	1382818..1383591	-	774	WP_000543891.1	C40 family peptidase	-
CEP72_RS07450	1383777..1384037	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
CEP72_RS07455	1384056..1384316	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
CEP72_RS07460	1384472..1385212	+	741	WP_001225679.1	murein L,D-transpeptidase	-
CEP72_RS07465	1385183..1385950	-	768	WP_001301698.1	class II glutamine amidotransferase	-
CEP72_RS07470	1386055..1386633	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T78268 WP_001303998.1 NZ_CP022050:1384056-1384316 [Escherichia coli O157]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T78268 NZ_CP022050:1384056-1384316 [Escherichia coli O157]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT78268 WP_000729705.1 NZ_CP022050:1383777-1384037 [Escherichia coli O157]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT78268 NZ_CP022050:1383777-1384037 [Escherichia coli O157]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA