Detailed information of TA system TA078267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1377659..1378353
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: CEP72_RS07415
• Protein ID: WP_001263495.1
• Coordinates: 1377659..1378057 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CEP72_RS07420
• Protein ID: WP_000554757.1
• Coordinates: 1378060..1378353 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1373246..1373326	-	81	NuclAT_11	-	-
-	1373246..1373326	-	81	NuclAT_11	-	-
-	1373246..1373326	-	81	NuclAT_11	-	-
-	1373246..1373326	-	81	NuclAT_11	-	-
CEP72_RS07390	1373922..1374380	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CEP72_RS07395	1374641..1376098	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CEP72_RS07400	1376155..1376676	-	522	Protein_1328	peptide chain release factor H	-
CEP72_RS07405	1376675..1376878	-	204	Protein_1329	RNA ligase RtcB family protein	-
CEP72_RS07410	1377197..1377649	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
CEP72_RS07415	1377659..1378057	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CEP72_RS07420	1378060..1378353	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CEP72_RS07425	1378405..1379460	-	1056	WP_001226186.1	DNA polymerase IV	-
CEP72_RS07430	1379531..1380316	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CEP72_RS07435	1380288..1382000	+	1713	Protein_1335	flagellar biosynthesis protein FlhA	-
CEP72_RS07440	1382145..1382642	-	498	Protein_1336	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T78267 WP_001263495.1 NZ_CP022050:c1378057-1377659 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T78267 NZ_CP022050:c1378057-1377659 [Escherichia coli O157]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT78267 WP_000554757.1 NZ_CP022050:c1378353-1378060 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT78267 NZ_CP022050:c1378353-1378060 [Escherichia coli O157]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6