Detailed information of TA system TA078262

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 491296..491521
• Replicon: chromosome
• Accession: NZ_CP022050
• Organism: Escherichia coli O157 strain FDAARGOS_293

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: CEP72_RS02955
• Protein ID: WP_000813263.1
• Coordinates: 491296..491451 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	491463..491521 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP72_RS30585	486750..487463	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
CEP72_RS02925	487601..487797	-	197	Protein_520	TrmB family transcriptional regulator	-
CEP72_RS02930	488084..488902	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
CEP72_RS02935	489054..489425	-	372	WP_000090264.1	antitermination protein	-
CEP72_RS02940	489415..489786	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
CEP72_RS02945	489799..490848	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
CEP72_RS02950	490850..491128	-	279	WP_001341388.1	hypothetical protein	-
CEP72_RS02955	491296..491451	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	491463..491521	+	59	-	-	Antitoxin
CEP72_RS02960	492056..492829	+	774	WP_000160654.1	alpha/beta hydrolase	-
CEP72_RS02965	493181..493594	-	414	WP_001151233.1	DUF977 family protein	-
CEP72_RS02970	493610..494380	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
CEP72_RS02975	494402..495148	-	747	WP_000788751.1	ATP-binding protein	-
CEP72_RS02980	495155..496246	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T78262 WP_000813263.1 NZ_CP022050:c491451-491296 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T78262 NZ_CP022050:c491451-491296 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT78262 NZ_CP022050:491463-491521 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin