Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2653268..2653917 Replicon chromosome
Accession NZ_CP022049
Organism Citrobacter braakii strain FDAARGOS_290

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag CEP69_RS13130 Protein ID WP_016157800.1
Coordinates 2653576..2653917 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag CEP69_RS13125 Protein ID WP_016157799.1
Coordinates 2653268..2653567 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP69_RS13100 2648604..2649422 - 819 WP_016157795.1 lipoprotein NlpA -
CEP69_RS13105 2649594..2650010 - 417 WP_016157796.1 GNAT family N-acetyltransferase -
CEP69_RS13110 2650141..2650434 + 294 WP_016155038.1 hypothetical protein -
CEP69_RS13115 2650527..2651909 + 1383 WP_016157797.1 glycoside hydrolase family 1 protein -
CEP69_RS13120 2651965..2653149 - 1185 WP_016157798.1 purine ribonucleoside efflux pump NepI -
CEP69_RS13125 2653268..2653567 - 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
CEP69_RS13130 2653576..2653917 - 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CEP69_RS13135 2654018..2654470 - 453 WP_016157801.1 DUF1198 family protein -
CEP69_RS13140 2654619..2656010 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
CEP69_RS13145 2656153..2657481 - 1329 WP_016155042.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T78240 WP_016157800.1 NZ_CP022049:c2653917-2653576 [Citrobacter braakii]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

>T78240 NZ_CP022049:c2653917-2653576 [Citrobacter braakii]
ATGTGGGAAGTGGAAACAACGGATGTTTTCGATAAATGGTTTGACGTACAGACCGAGGCGCTCAAGGAAGATATGCTCGC
AGCGATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTGCAGCATCAGGGCAACCCGATTCGGGCGTTCTTCGTGTTTGATCCTTCTCGCCATGGCATC
GTGTTATGCGCAGGCGATAAAACAGGGTTAAACGAGAAGAAATTCTATAAAGAGATGATCAGGCTTGCTGACGCTGAGTA
CCGAAATCACCTTAATAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT78240 WP_016157799.1 NZ_CP022049:c2653567-2653268 [Citrobacter braakii]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp

>AT78240 NZ_CP022049:c2653567-2653268 [Citrobacter braakii]
ATGGCGACGCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGCATTGCTGAAAAAGTAGAAGTGCTACG
TCAGGTGATTGCGTTAAATATGTTACGGGAAGAACTGAATCTCTCTCAGGCGGAACTTGCCTCGGCAATGGGCGTTAAGC
AGCCAACAATCGCAAAAATGGAACAGGCGGATAACGATCCTCGTCTGTCGACGCTTAAGCGTTATGTCACAGCGTTAGGT
GGCGAACTGAGCATTGATGTTAAGTTACCGACAGGTAAAAGAGTCGCGTTTCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References