Detailed information of TA system TA078225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 260104..260674
• Replicon: chromosome
• Accession: NZ_CP022038
• Organism: Jonesia denitrificans strain FDAARGOS_301

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C7R1W2
• Locus tag: CEP80_RS01320
• Protein ID: WP_015771058.1
• Coordinates: 260393..260674 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEP80_RS01315
• Protein ID: WP_015771059.1
• Coordinates: 260104..260406 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP80_RS01280	256022..256863	-	842	Protein_246	NAD(P)-binding protein	-
CEP80_RS01285	257333..257986	-	654	WP_015771065.1	hypothetical protein	-
CEP80_RS01290	258108..258362	-	255	WP_015771064.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CEP80_RS01295	258469..258789	+	321	WP_143713239.1	hypothetical protein	-
CEP80_RS01300	258791..259078	+	288	WP_015771062.1	transcriptional regulator	-
CEP80_RS01305	259116..259502	+	387	WP_015771061.1	hypothetical protein	-
CEP80_RS01310	259592..260107	-	516	WP_015771060.1	GNAT family N-acetyltransferase	-
CEP80_RS01315	260104..260406	-	303	WP_015771059.1	HigA family addiction module antidote protein	Antitoxin
CEP80_RS01320	260393..260674	-	282	WP_015771058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEP80_RS01325	260874..261119	+	246	WP_015771057.1	hypothetical protein	-
CEP80_RS01330	261116..262105	+	990	WP_015771056.1	SRPBCC domain-containing protein	-
CEP80_RS01335	262210..262545	+	336	WP_015771055.1	hypothetical protein	-
CEP80_RS01340	262636..262863	+	228	WP_015771054.1	hypothetical protein	-
CEP80_RS01345	262899..263261	+	363	WP_015771053.1	hypothetical protein	-
CEP80_RS01350	263402..263854	+	453	WP_015771052.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CEP80_RS13165	264282..264479	+	198	WP_015771050.1	hypothetical protein	-
CEP80_RS01360	264472..264816	+	345	WP_015771049.1	hypothetical protein	-
CEP80_RS01365	264862..265266	+	405	WP_041288145.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	252601..271307	18706

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10927.43 Da
• Isoelectric Point: 10.9360

>T78225 WP_015771058.1 NZ_CP022038:c260674-260393 [Jonesia denitrificans]
VIRSFGDKETERLWRRERVRSIDPRIHRVALRKLRQVGSAESIEDLRVPPGNRLEALKGNRSGQHSIRINDQWRICFVWT
AAGPEEVQIVDYH

Nucleotide

• Length: 282 bp

>T78225 NZ_CP022038:c260674-260393 [Jonesia denitrificans]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAGCGCGTGCGCTCGATCGATCCGAGGATTCA
TCGGGTCGCGCTGCGCAAACTGCGTCAGGTGGGGTCCGCGGAGTCCATCGAGGACCTCCGTGTCCCACCCGGTAACCGCC
TCGAAGCTCTCAAGGGCAACCGATCTGGTCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GCCGCCGGACCAGAGGAGGTGCAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11063.60 Da
• Isoelectric Point: 4.8129

>AT78225 WP_015771059.1 NZ_CP022038:c260406-260104 [Jonesia denitrificans]
MTTTDKIEPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLAKYFGTSAEFWINLQSHYEL
DRAEDLAGEQIASITPLQVA

Nucleotide

• Length: 303 bp

>AT78225 NZ_CP022038:c260406-260104 [Jonesia denitrificans]
TTGACTACCACTGACAAGATCGAGCCGATCCACCCGGGCGAGGTGCTCATGGAGGACTTCATCGAGGGCTTCGGCATCAC
GCAGAACAAGCTCGCCGTATCCATCGGCGTTCCGCCGCGACGGATCAACGAGATCGTGCACGGCAAGCGAGGGATCACCG
CAGACACGGCGCTGCGGCTCGCGAAGTACTTCGGCACCTCGGCCGAGTTCTGGATCAACCTGCAGAGCCACTACGAGCTG
GACCGTGCGGAGGACCTCGCGGGCGAGCAGATCGCGTCGATCACGCCACTGCAGGTCGCGTGA

Structures

Toxin

• AlphaFold DB: C7R1W2