Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3246263..3246860 Replicon chromosome
Accession NZ_CP022023
Organism Klebsiella pneumoniae strain 19051

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag CDA51_RS16160 Protein ID WP_004142563.1
Coordinates 3246263..3246580 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag CDA51_RS16165 Protein ID WP_004142561.1
Coordinates 3246573..3246860 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDA51_RS16140 3241964..3243391 - 1428 WP_009308097.1 MFS transporter -
CDA51_RS16145 3243500..3244366 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
CDA51_RS16155 3245035..3246078 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
CDA51_RS16160 3246263..3246580 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CDA51_RS16165 3246573..3246860 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
CDA51_RS16170 3247125..3247778 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
CDA51_RS16175 3247898..3248266 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
CDA51_RS16180 3248266..3248837 - 572 Protein_3047 TetR/AcrR family transcriptional regulator -
CDA51_RS16185 3249017..3250132 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
CDA51_RS16190 3250163..3250504 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
CDA51_RS16195 3250522..3250770 - 249 WP_020325243.1 DUF1158 domain-containing protein -
CDA51_RS27125 3251273..3251503 + 231 WP_198360451.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T78211 WP_004142563.1 NZ_CP022023:3246263-3246580 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T78211 NZ_CP022023:3246263-3246580 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT78211 WP_004142561.1 NZ_CP022023:3246573-3246860 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT78211 NZ_CP022023:3246573-3246860 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References