Detailed information of TA system TA078088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2317713..2318394
• Replicon: chromosome
• Accession: NZ_CP022002
• Organism: Pseudomonas aeruginosa strain Pa1242

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CD799_RS10980
• Protein ID: WP_015503432.1
• Coordinates: 2317713..2318078 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CD799_RS10985
• Protein ID: WP_016561576.1
• Coordinates: 2318071..2318394 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD799_RS10950	2313718..2314152	-	435	WP_003158601.1	RidA family protein	-
CD799_RS10955	2314373..2315326	+	954	WP_003085661.1	LysR family transcriptional regulator	-
CD799_RS10960	2315299..2316183	-	885	WP_019725847.1	LysR family transcriptional regulator	-
CD799_RS10965	2316284..2316709	+	426	WP_003163196.1	VOC family protein	-
CD799_RS10970	2316728..2317000	-	273	WP_003085667.1	hypothetical protein	-
CD799_RS10975	2317207..2317449	+	243	WP_043884955.1	hypothetical protein	-
CD799_RS10980	2317713..2318078	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CD799_RS10985	2318071..2318394	+	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
CD799_RS33695	2318510..2318629	-	120	Protein_2130	IS5/IS1182 family transposase	-
CD799_RS10990	2318684..2319016	+	333	WP_088376638.1	hypothetical protein	-
CD799_RS10995	2319016..2319291	+	276	WP_004353146.1	hypothetical protein	-
CD799_RS11000	2319465..2320019	+	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
CD799_RS33865	2320236..2320487	+	252	WP_124136459.1	hypothetical protein	-
CD799_RS33870	2320517..2321383	+	867	WP_023081971.1	hypothetical protein	-
CD799_RS11010	2321991..2322434	-	444	WP_014603615.1	SocA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2316284..2362673	46389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T78088 WP_015503432.1 NZ_CP022002:2317713-2318078 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T78088 NZ_CP022002:2317713-2318078 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT78088 WP_016561576.1 NZ_CP022002:2318071-2318394 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT78088 NZ_CP022002:2318071-2318394 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA