Detailed information of TA system TA078084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 5333071..5333846
• Replicon: chromosome
• Accession: NZ_CP022001
• Organism: Pseudomonas aeruginosa strain Pa1207

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V6ADY6
• Locus tag: CD800_RS25655
• Protein ID: WP_009518525.1
• Coordinates: 5333071..5333529 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V6AEP7
• Locus tag: CD800_RS25660
• Protein ID: WP_023098543.1
• Coordinates: 5333529..5333846 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD800_RS25630	5328412..5329359	+	948	WP_009518520.1	TIGR03756 family integrating conjugative element protein	-
CD800_RS25635	5329369..5330763	+	1395	WP_009518521.1	integrating conjugative element protein	-
CD800_RS25640	5330760..5331119	+	360	WP_009518522.1	hypothetical protein	-
CD800_RS25645	5331134..5332651	+	1518	WP_009518523.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
CD800_RS25650	5332667..5333044	-	378	WP_009518524.1	DUF3742 family protein	-
CD800_RS25655	5333071..5333529	-	459	WP_009518525.1	type II toxin-antitoxin system YhaV family toxin	Toxin
CD800_RS25660	5333529..5333846	-	318	WP_023098543.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
CD800_RS25665	5334146..5335963	+	1818	WP_023098542.1	TraI domain-containing protein	-
CD800_RS36140	5335945..5336091	-	147	WP_023098541.1	MerR family DNA-binding transcriptional regulator	-
CD800_RS25675	5336240..5337151	-	912	WP_023098540.1	NAD(P)H-binding protein	-
CD800_RS25680	5337288..5338676	+	1389	Protein_4972	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5333071..5354103	21032
	-	inside	Integrative and Conjugative Element	-	-	5150139..5332109	181970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (8-144)

Antitoxin

• PrlF antitoxin (8-100)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17660.02 Da
• Isoelectric Point: 10.1848

>T78084 WP_009518525.1 NZ_CP022001:c5333529-5333071 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Nucleotide

• Length: 459 bp

>T78084 NZ_CP022001:c5333529-5333071 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11170.72 Da
• Isoelectric Point: 4.7182

>AT78084 WP_023098543.1 NZ_CP022001:c5333846-5333529 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Nucleotide

• Length: 318 bp

>AT78084 NZ_CP022001:c5333846-5333529 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGCTGACCTCTAAAGGCCAGATCACGCTGCCCAAGCCCATCCGGCAGGCGCTCGG
CGTCGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Structures

Toxin

• AlphaFold DB: V6ADY6

Antitoxin

• AlphaFold DB: V6AEP7