Detailed information of TA system TA078021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4873672..4874269
• Replicon: chromosome
• Accession: NZ_CP021960
• Organism: Klebsiella pneumoniae strain AR_0139

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: AM386_RS26160
• Protein ID: WP_004893639.1
• Coordinates: 4873952..4874269 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: AM386_RS26155
• Protein ID: WP_004142561.1
• Coordinates: 4873672..4873959 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM386_RS26125	4869879..4870127	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
AM386_RS26130	4870145..4870486	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
AM386_RS26135	4870517..4871632	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
AM386_RS26140	4871812..4872393	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
AM386_RS26145	4872393..4872761	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
AM386_RS26150	4872881..4873534	+	654	Protein_4722	oxygen-insensitive NAD(P)H nitroreductase	-
AM386_RS26155	4873672..4873959	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
AM386_RS26160	4873952..4874269	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM386_RS26165	4874454..4875497	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
AM386_RS26175	4876167..4877033	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
AM386_RS26180	4877142..4878569	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T78021 WP_004893639.1 NZ_CP021960:c4874269-4873952 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T78021 NZ_CP021960:c4874269-4873952 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT78021 WP_004142561.1 NZ_CP021960:c4873959-4873672 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT78021 NZ_CP021960:c4873959-4873672 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1