Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 1272..1902 Replicon plasmid tig00001025alt_u_pilon
Accession NZ_CP021954
Organism Klebsiella pneumoniae strain AR_0148

Toxin (Protein)


Gene name higB Uniprot ID A0A3V9PY09
Locus tag AM395_RS29465 Protein ID WP_001708474.1
Coordinates 1567..1902 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A704FLZ1
Locus tag AM395_RS29460 Protein ID WP_001708475.1
Coordinates 1272..1562 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM395_RS29455 559..1212 - 654 WP_001708476.1 hypothetical protein -
AM395_RS29460 1272..1562 - 291 WP_001708475.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
AM395_RS29465 1567..1902 - 336 WP_001708474.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM395_RS29470 2107..2775 - 669 WP_044296050.1 hypothetical protein -
AM395_RS29475 2825..3151 - 327 WP_044296048.1 hypothetical protein -
AM395_RS29480 3464..4024 - 561 WP_044296046.1 hypothetical protein -
AM395_RS29950 4042..4392 - 351 WP_001708471.1 hypothetical protein -
AM395_RS29485 4427..4762 - 336 WP_044296044.1 hypothetical protein -
AM395_RS29490 4759..5028 - 270 WP_024172976.1 hypothetical protein -
AM395_RS29495 5042..5419 - 378 WP_024172975.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..42816 42816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-104)

Antitoxin

Antitoxin MqsA/HigA-2

(21-94)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12615.30 Da        Isoelectric Point: 5.2407

>T77987 WP_001708474.1 NZ_CP021954:c1902-1567 [Klebsiella pneumoniae]
MEYYEFVETSVFTREMKTLLSDDEYKEFQTFLIENPEAGDLIVGTGGCRKVRWSRQGTGKSSGVRAIYYFYNPAGRLYML
IIYPKSEKDSLTAAEKNQLKAVVAGFKGEEG

Download         Length: 336 bp

>T77987 NZ_CP021954:c1902-1567 [Klebsiella pneumoniae]
ATGGAATACTACGAGTTTGTAGAAACCTCTGTGTTCACGCGAGAGATGAAAACTCTACTCAGTGATGATGAATACAAAGA
GTTTCAAACGTTCCTCATAGAGAACCCGGAGGCGGGTGATCTGATTGTAGGAACTGGCGGTTGTCGCAAGGTAAGATGGT
CACGGCAGGGAACAGGTAAAAGTTCAGGTGTCCGGGCTATTTACTACTTCTACAATCCAGCAGGCCGCTTATACATGCTT
ATCATCTATCCGAAGAGTGAGAAGGATAGTTTGACGGCAGCAGAGAAAAACCAGCTCAAAGCTGTTGTAGCGGGTTTTAA
AGGTGAAGAGGGCTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10909.62 Da        Isoelectric Point: 10.0073

>AT77987 WP_001708475.1 NZ_CP021954:c1562-1272 [Klebsiella pneumoniae]
MRKELFDDLIASAKEAVEIHQGLREPARVTRFERPDVKAIRAKVHMKQDDFASLIGVSSSLVQSWEQGLRTPAGAALKLL
RVIERKPEFVQELREA

Download         Length: 291 bp

>AT77987 NZ_CP021954:c1562-1272 [Klebsiella pneumoniae]
ATGCGTAAAGAGCTTTTTGATGATCTGATTGCTTCTGCTAAAGAAGCTGTAGAAATCCATCAGGGTTTACGTGAGCCAGC
TCGCGTGACCCGTTTTGAACGTCCAGATGTTAAAGCTATTCGGGCTAAGGTTCATATGAAACAAGATGATTTTGCCAGCC
TGATCGGGGTTAGCTCTTCTCTTGTTCAGTCATGGGAACAGGGGTTAAGAACTCCGGCAGGTGCAGCGTTAAAGCTGTTG
CGTGTCATCGAGCGTAAACCTGAATTTGTTCAGGAACTACGTGAAGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V9PY09

Antitoxin

Source ID Structure
AlphaFold DB A0A704FLZ1

References