Detailed information of TA system TA077986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 102400..103057
• Replicon: plasmid tig00000169_pilon
• Accession: NZ_CP021952
• Organism: Klebsiella pneumoniae strain AR_0148

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM395_RS28525
• Protein ID: WP_000270043.1
• Coordinates: 102707..103057 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM395_RS28520
• Protein ID: WP_000124640.1
• Coordinates: 102400..102702 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM395_RS28470	98034..98462	+	429	WP_000591076.1	hypothetical protein	-
AM395_RS28475	98520..98879	+	360	WP_000422769.1	hypothetical protein	-
AM395_RS28480	98879..99325	+	447	WP_000919343.1	hypothetical protein	-
AM395_RS28485	99322..99840	+	519	WP_000210757.1	nitrite reductase	-
AM395_RS28490	99840..100070	+	231	WP_000972665.1	hypothetical protein	-
AM395_RS28495	100057..100914	+	858	WP_001167036.1	hypothetical protein	-
AM395_RS30585	100826..101131	+	306	WP_171820680.1	hypothetical protein	-
AM395_RS28505	101134..101661	+	528	WP_004201083.1	thermonuclease family protein	-
AM395_RS28510	101719..101991	+	273	WP_001043046.1	HU family DNA-binding protein	-
AM395_RS28515	102080..102373	+	294	WP_001239998.1	hypothetical protein	-
AM395_RS28520	102400..102702	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM395_RS28525	102707..103057	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM395_RS28530	103220..103768	+	549	WP_001061573.1	hypothetical protein	-
AM395_RS28540	104109..104303	+	195	WP_000343597.1	hypothetical protein	-
AM395_RS28545	104314..104685	+	372	WP_000516918.1	hypothetical protein	-
AM395_RS28550	104678..105148	+	471	WP_001281821.1	hypothetical protein	-
AM395_RS28555	105163..105498	-	336	WP_000683477.1	hypothetical protein	-
AM395_RS28560	105595..106083	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM395_RS28565	106086..106583	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / qacE / sul1 / blaSHV-182 / aac(3)-IId / blaNDM-1 / rmtC / qnrB9 / blaCMY-6	-	1..176349	176349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77986 WP_000270043.1 NZ_CP021952:c103057-102707 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77986 NZ_CP021952:c103057-102707 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77986 WP_000124640.1 NZ_CP021952:c102702-102400 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77986 NZ_CP021952:c102702-102400 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9