Detailed information of TA system TA077923

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69062..69488
• Replicon: plasmid unitig_4
• Accession: NZ_CP021938
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM434_RS29225
• Protein ID: WP_001312861.1
• Coordinates: 69062..69220 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69264..69488 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS29185	64148..64387	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
AM434_RS29190	64495..65142	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
AM434_RS29195	65334..65717	-	384	WP_001151529.1	relaxosome protein TraM	-
AM434_RS29200	66050..66640	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
AM434_RS29205	66937..67758	-	822	WP_001234445.1	DUF945 domain-containing protein	-
AM434_RS29210	67869..68165	-	297	WP_001272251.1	hypothetical protein	-
AM434_RS29965	68557..68761	+	205	Protein_92	pilus protein	-
AM434_RS29225	69062..69220	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	69264..69488	-	225	NuclAT_0	-	Antitoxin
-	69264..69488	-	225	NuclAT_0	-	Antitoxin
-	69264..69488	-	225	NuclAT_0	-	Antitoxin
-	69264..69488	-	225	NuclAT_0	-	Antitoxin
AM434_RS29230	69300..69479	+	180	WP_001309233.1	hypothetical protein	-
AM434_RS29235	69500..70219	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
AM434_RS29240	70216..70650	-	435	WP_052983109.1	conjugation system SOS inhibitor PsiB	-
AM434_RS29245	70719..72683	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
AM434_RS29250	72744..72977	-	234	WP_033551487.1	DUF905 family protein	-
AM434_RS29255	73035..73562	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
AM434_RS29265	73864..74313	+	450	WP_088251387.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..75318	75318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77923 WP_001312861.1 NZ_CP021938:c69220-69062 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77923 NZ_CP021938:c69220-69062 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT77923 NZ_CP021938:c69488-69264 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin