Detailed information of TA system TA077919

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26470..26723
• Replicon: plasmid unitig_4
• Accession: NZ_CP021938
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: AM434_RS28950
• Protein ID: WP_001312851.1
• Coordinates: 26470..26619 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26664..26723 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS28900	21813..22295	-	483	WP_001311056.1	hypothetical protein	-
AM434_RS28905	22412..23260	-	849	WP_032256585.1	3'-5' exonuclease	-
AM434_RS28910	23306..23587	-	282	WP_000970000.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AM434_RS28915	23584..23853	-	270	WP_000079928.1	hypothetical protein	-
AM434_RS28930	24768..25625	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
AM434_RS28935	25618..25692	-	75	WP_001365705.1	RepA leader peptide Tap	-
AM434_RS28945	25937..26185	-	249	WP_000083839.1	replication regulatory protein RepA	-
AM434_RS28950	26470..26619	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	26664..26723	+	60	NuclAT_1	-	Antitoxin
-	26664..26723	+	60	NuclAT_1	-	Antitoxin
-	26664..26723	+	60	NuclAT_1	-	Antitoxin
-	26664..26723	+	60	NuclAT_1	-	Antitoxin
AM434_RS28960	26866..27339	-	474	WP_016240489.1	hypothetical protein	-
AM434_RS28965	27494..28084	-	591	WP_042018152.1	DUF2726 domain-containing protein	-
AM434_RS28970	28122..28331	-	210	WP_001369435.1	hemolysin expression modulator Hha	-
AM434_RS28975	28377..28838	-	462	WP_016230918.1	thermonuclease family protein	-
AM434_RS28980	29083..29295	-	213	WP_001309245.1	ANR family transcriptional regulator	-
AM434_RS28985	29424..29984	-	561	WP_000139321.1	fertility inhibition protein FinO	-
AM434_RS28990	30087..30947	-	861	WP_000704524.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..75318	75318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T77919 WP_001312851.1 NZ_CP021938:c26619-26470 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T77919 NZ_CP021938:c26619-26470 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT77919 NZ_CP021938:26664-26723 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin