Detailed information of TA system TA077912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 103632..104289
• Replicon: plasmid unitig_2
• Accession: NZ_CP021936
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM434_RS27365
• Protein ID: WP_000270043.1
• Coordinates: 103939..104289 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM434_RS27360
• Protein ID: WP_000124640.1
• Coordinates: 103632..103934 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS27310	99266..99694	+	429	WP_000591076.1	hypothetical protein	-
AM434_RS27315	99752..100111	+	360	WP_000422769.1	hypothetical protein	-
AM434_RS27320	100111..100557	+	447	WP_000919343.1	hypothetical protein	-
AM434_RS27325	100554..101072	+	519	WP_000210757.1	nitrite reductase	-
AM434_RS27330	101072..101302	+	231	WP_000972665.1	hypothetical protein	-
AM434_RS27335	101289..102146	+	858	WP_001167036.1	hypothetical protein	-
AM434_RS29900	102058..102363	+	306	WP_171820680.1	hypothetical protein	-
AM434_RS27345	102366..102893	+	528	WP_004201083.1	thermonuclease family protein	-
AM434_RS27350	102951..103223	+	273	WP_001043046.1	HU family DNA-binding protein	-
AM434_RS27355	103312..103605	+	294	WP_001239998.1	hypothetical protein	-
AM434_RS27360	103632..103934	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM434_RS27365	103939..104289	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM434_RS27370	104452..105000	+	549	WP_001061573.1	hypothetical protein	-
AM434_RS27380	105341..105535	+	195	WP_000343597.1	hypothetical protein	-
AM434_RS27385	105546..105917	+	372	WP_000516918.1	hypothetical protein	-
AM434_RS27390	105910..106380	+	471	WP_001281821.1	hypothetical protein	-
AM434_RS27395	106395..106730	-	336	WP_000683477.1	hypothetical protein	-
AM434_RS27400	106827..107315	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM434_RS27405	107318..107815	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC / blaNDM-1	htpB	1..138340	138340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77912 WP_000270043.1 NZ_CP021936:c104289-103939 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77912 NZ_CP021936:c104289-103939 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77912 WP_000124640.1 NZ_CP021936:c103934-103632 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77912 NZ_CP021936:c103934-103632 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9