Detailed information of TA system TA077896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2410644..2411246
• Replicon: chromosome
• Accession: NZ_CP021935
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AM434_RS12215
• Protein ID: WP_000897302.1
• Coordinates: 2410644..2410955 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM434_RS12220
• Protein ID: WP_000356397.1
• Coordinates: 2410956..2411246 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS12190	2406558..2407157	+	600	WP_001296610.1	glucose-1-phosphatase	-
AM434_RS12195	2407151..2408023	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AM434_RS12200	2408020..2408457	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM434_RS12205	2408502..2409443	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM434_RS12210	2409507..2410415	-	909	WP_001385591.1	alpha/beta hydrolase	-
AM434_RS12215	2410644..2410955	+	312	WP_000897302.1	hypothetical protein	Toxin
AM434_RS12220	2410956..2411246	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM434_RS12230	2411605..2411883	+	279	WP_001296612.1	hypothetical protein	-
AM434_RS12240	2412280..2412498	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AM434_RS12245	2412683..2413132	-	450	WP_032140890.1	hypothetical protein	-
AM434_RS12250	2413448..2414296	-	849	WP_001038650.1	hypothetical protein	-
AM434_RS12255	2414586..2414828	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AM434_RS12260	2415010..2415939	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T77896 WP_000897302.1 NZ_CP021935:2410644-2410955 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T77896 NZ_CP021935:2410644-2410955 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT77896 WP_000356397.1 NZ_CP021935:2410956-2411246 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT77896 NZ_CP021935:2410956-2411246 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416