77893

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1559710..1560437 Replicon chromosome
Accession NZ_CP021935
Organism Escherichia coli strain AR_0055

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AM434_RS07855 Protein ID WP_000550189.1
Coordinates 1560123..1560437 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AM434_RS07850 Protein ID WP_000560269.1
Coordinates 1559710..1560126 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM434_RS07840 1555070..1557421 + 2352 WP_000695432.1 alpha-glucosidase -
AM434_RS07845 1557647..1559665 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AM434_RS07850 1559710..1560126 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AM434_RS07855 1560123..1560437 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AM434_RS07865 1560722..1561858 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AM434_RS07870 1561943..1562446 + 504 WP_001295542.1 M48 family metallopeptidase -
AM434_RS07875 1562523..1563215 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
AM434_RS07880 1563294..1564280 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T77893 WP_000550189.1 NZ_CP021935:c1560437-1560123 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T77893 NZ_CP021935:c1560437-1560123 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT77893 WP_000560269.1 NZ_CP021935:c1560126-1559710 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT77893 NZ_CP021935:c1560126-1559710 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References