Detailed information of TA system TA077795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 890965..891695
• Replicon: chromosome
• Accession: NZ_CP021896
• Organism: Enterobacter cloacae complex sp. strain AR_0050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: AM429_RS04360
• Protein ID: WP_022646552.1
• Coordinates: 890965..891279 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM429_RS04365
• Protein ID: WP_022646553.1
• Coordinates: 891276..891695 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM429_RS04335 (AM429_04305)	886631..887716	+	1086	Protein_804	cellulase family glycosylhydrolase	-
AM429_RS04340 (AM429_04310)	887622..888806	-	1185	WP_022646548.1	multidrug efflux MFS transporter EmrD	-
AM429_RS04345 (AM429_04315)	888982..889815	-	834	WP_022646549.1	DMT family transporter	-
AM429_RS04350 (AM429_04320)	889878..890324	-	447	WP_022646550.1	GNAT family N-acetyltransferase	-
AM429_RS04355 (AM429_04325)	890389..890478	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
AM429_RS04360 (AM429_04330)	890965..891279	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM429_RS04365 (AM429_04335)	891276..891695	+	420	WP_022646553.1	helix-turn-helix domain-containing protein	Antitoxin
AM429_RS04370 (AM429_04340)	891842..891940	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
AM429_RS04375 (AM429_04345)	892170..893858	+	1689	WP_022646554.1	acetolactate synthase large subunit	-
AM429_RS04380 (AM429_04350)	893862..894149	+	288	WP_015570113.1	acetolactate synthase small subunit	-
AM429_RS04385 (AM429_04355)	894275..894868	+	594	WP_022646555.1	transcriptional regulator UhpA	-
AM429_RS04390 (AM429_04360)	894865..896370	+	1506	WP_022646556.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T77795 WP_022646552.1 NZ_CP021896:890965-891279 [Enterobacter cloacae complex sp.]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T77795 NZ_CP021896:890965-891279 [Enterobacter cloacae complex sp.]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15480.10 Da
• Isoelectric Point: 5.6761

>AT77795 WP_022646553.1 NZ_CP021896:891276-891695 [Enterobacter cloacae complex sp.]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT77795 NZ_CP021896:891276-891695 [Enterobacter cloacae complex sp.]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A155SSD8