Detailed information of TA system TA077794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 777782..778384
• Replicon: chromosome
• Accession: NZ_CP021896
• Organism: Enterobacter cloacae complex sp. strain AR_0050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: AM429_RS03800
• Protein ID: WP_022649837.1
• Coordinates: 778073..778384 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM429_RS03795
• Protein ID: WP_022649836.1
• Coordinates: 777782..778072 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM429_RS03780 (AM429_03755)	775280..776182	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
AM429_RS03785 (AM429_03760)	776179..776814	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
AM429_RS03790 (AM429_03765)	776811..777740	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
AM429_RS03795 (AM429_03770)	777782..778072	-	291	WP_022649836.1	NadS family protein	Antitoxin
AM429_RS03800 (AM429_03775)	778073..778384	-	312	WP_022649837.1	hypothetical protein	Toxin
AM429_RS03805 (AM429_03780)	778533..779474	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
AM429_RS03810 (AM429_03785)	779519..779956	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
AM429_RS03815 (AM429_03790)	779953..780834	-	882	WP_022649840.1	virulence factor BrkB family protein	-
AM429_RS03820 (AM429_03795)	780828..781427	-	600	WP_022649841.1	glucose-1-phosphatase	-
AM429_RS03825 (AM429_03800)	781546..782346	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM429_RS03830 (AM429_03805)	782381..783277	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T77794 WP_022649837.1 NZ_CP021896:c778384-778073 [Enterobacter cloacae complex sp.]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T77794 NZ_CP021896:c778384-778073 [Enterobacter cloacae complex sp.]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT77794 WP_022649836.1 NZ_CP021896:c778072-777782 [Enterobacter cloacae complex sp.]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT77794 NZ_CP021896:c778072-777782 [Enterobacter cloacae complex sp.]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6