Detailed information of TA system TA077783

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66579..66843
• Replicon: plasmid tig00001287_pilon
• Accession: NZ_CP021882
• Organism: Escherichia coli strain AR_0137

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: AM384_RS26545
• Protein ID: WP_001387489.1
• Coordinates: 66579..66731 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	66783..66843 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM384_RS26975	62144..62260	+	117	Protein_72	IncI1-type conjugal transfer lipoprotein TraH	-
AM384_RS26520	62259..64013	+	1755	Protein_73	DotA/TraY family protein	-
AM384_RS26525	64084..64746	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
AM384_RS26530	64818..65027	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AM384_RS27195	65419..65595	+	177	WP_001054897.1	hypothetical protein	-
AM384_RS26535	65660..65956	-	297	WP_011264046.1	hypothetical protein	-
AM384_RS26540	66256..66507	+	252	WP_001291964.1	hypothetical protein	-
AM384_RS26545	66579..66731	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	66783..66843	+	61	NuclAT_0	-	Antitoxin
-	66783..66843	+	61	NuclAT_0	-	Antitoxin
-	66783..66843	+	61	NuclAT_0	-	Antitoxin
-	66783..66843	+	61	NuclAT_0	-	Antitoxin
AM384_RS26550	67046..67393	+	348	Protein_80	protein finQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..67973	67973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T77783 WP_001387489.1 NZ_CP021882:c66731-66579 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T77783 NZ_CP021882:c66731-66579 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT77783 NZ_CP021882:66783-66843 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin