Detailed information of TA system TA077779

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92377..92631
• Replicon: plasmid tig00001069_pilon
• Accession: NZ_CP021880
• Organism: Escherichia coli strain AR_0137

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM384_RS24950
• Protein ID: WP_015387399.1
• Coordinates: 92425..92631 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	92377..92438 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM384_RS24900	88029..88241	+	213	WP_005012601.1	hypothetical protein	-
AM384_RS27160	88542..88631	-	90	Protein_101	IS1 family transposase	-
AM384_RS24915	88686..89363	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
AM384_RS24920	89363..89710	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM384_RS24925	89730..91301	+	1572	WP_023149734.1	IS66 family transposase	-
AM384_RS24930	91339..91955	-	617	Protein_105	IS1 family transposase	-
AM384_RS24940	92056..92238	+	183	WP_000968309.1	hypothetical protein	-
-	92377..92438	-	62	NuclAT_2	-	Antitoxin
-	92377..92438	-	62	NuclAT_2	-	Antitoxin
-	92377..92438	-	62	NuclAT_2	-	Antitoxin
-	92377..92438	-	62	NuclAT_2	-	Antitoxin
AM384_RS24950	92425..92631	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM384_RS24955	92915..93172	+	258	WP_000083833.1	replication regulatory protein RepA	-
AM384_RS24965	93408..93482	+	75	WP_032336874.1	RepA leader peptide Tap	-
AM384_RS24970	93475..93921	+	447	Protein_110	incFII family plasmid replication initiator RepA	-
AM384_RS24975	93867..94349	-	483	Protein_111	VENN motif pre-toxin domain-containing protein	-
AM384_RS24980	95242..96462	+	1221	WP_000410951.1	arginine deiminase	-
AM384_RS24985	96473..97384	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / aadA5 / qacE / sul1 / mph(A) / tet(B)	iucA / iucB / iucC / iucD / iutA	1..169449	169449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T77779 WP_015387399.1 NZ_CP021880:92425-92631 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T77779 NZ_CP021880:92425-92631 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT77779 NZ_CP021880:c92438-92377 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin