Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3170033..3170535 Replicon chromosome
Accession NZ_CP021879
Organism Escherichia coli strain AR_0137

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM384_RS16020 Protein ID WP_000767829.1
Coordinates 3170281..3170535 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM384_RS16015 Protein ID WP_001259255.1
Coordinates 3170033..3170284 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM384_RS15985 3165211..3166278 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM384_RS15990 3166278..3167348 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
AM384_RS15995 3167345..3168649 - 1305 WP_001446927.1 histidinol dehydrogenase -
AM384_RS16000 3168655..3169554 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
AM384_RS16010 3169700..3169750 - 51 WP_001364200.1 his operon leader peptide -
AM384_RS16015 3170033..3170284 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM384_RS16020 3170281..3170535 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM384_RS16025 3170618..3171442 + 825 WP_000754737.1 SDR family oxidoreductase -
AM384_RS16030 3171488..3172417 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM384_RS26725 3172632..3172694 + 63 WP_010723108.1 membrane protein YoeI -
AM384_RS16035 3172684..3174042 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM384_RS16040 3174221..3175279 + 1059 WP_000492324.1 transport protein YeeE -
AM384_RS16045 3175293..3175520 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77761 WP_000767829.1 NZ_CP021879:3170281-3170535 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77761 NZ_CP021879:3170281-3170535 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77761 WP_001259255.1 NZ_CP021879:3170033-3170284 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77761 NZ_CP021879:3170033-3170284 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References