Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 228555..229249 Replicon chromosome
Accession NZ_CP021879
Organism Escherichia coli strain AR_0137

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag AM384_RS01225 Protein ID WP_001263489.1
Coordinates 228555..228953 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AM384_RS01230 Protein ID WP_000554758.1
Coordinates 228956..229249 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 224143..224223 - 81 NuclAT_12 - -
- 224143..224223 - 81 NuclAT_12 - -
- 224143..224223 - 81 NuclAT_12 - -
- 224143..224223 - 81 NuclAT_12 - -
AM384_RS01195 224819..225277 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
AM384_RS01200 225538..226995 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
AM384_RS01205 227052..227573 - 522 Protein_214 peptide chain release factor H -
AM384_RS01210 227569..227775 - 207 Protein_215 RtcB family protein -
AM384_RS01220 228093..228545 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
AM384_RS01225 228555..228953 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM384_RS01230 228956..229249 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM384_RS01235 229301..230356 - 1056 WP_001226164.1 DNA polymerase IV -
AM384_RS01240 230427..231212 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
AM384_RS01245 231184..232896 + 1713 Protein_221 flagellar biosynthesis protein FlhA -
AM384_RS01250 233120..233617 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 201804..234419 32615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T77750 WP_001263489.1 NZ_CP021879:c228953-228555 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T77750 NZ_CP021879:c228953-228555 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT77750 WP_000554758.1 NZ_CP021879:c229249-228956 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT77750 NZ_CP021879:c229249-228956 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References