Detailed information of TA system TA077741

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83820..84062
• Replicon: plasmid pH105
• Accession: NZ_CP021871
• Organism: Escherichia coli strain H105

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CCL28_RS26295
• Protein ID: WP_001312861.1
• Coordinates: 83820..83978 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84022..84062 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL28_RS26255	78932..79159	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CCL28_RS26260	79247..79924	-	678	WP_001348626.1	PAS domain-containing protein	-
CCL28_RS26265	80058..80441	-	384	WP_001151566.1	relaxosome protein TraM	-
CCL28_RS26270	80772..81374	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CCL28_RS26275	81671..82492	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CCL28_RS26280	82611..82898	-	288	WP_000107535.1	hypothetical protein	-
CCL28_RS27200	82923..83129	-	207	WP_000275859.1	hypothetical protein	-
CCL28_RS27205	83042..83377	-	336	WP_013023876.1	hypothetical protein	-
CCL28_RS26295	83820..83978	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84022..84062	-	41	NuclAT_1	-	Antitoxin
-	84022..84062	-	41	NuclAT_1	-	Antitoxin
-	84022..84062	-	41	NuclAT_1	-	Antitoxin
-	84022..84062	-	41	NuclAT_1	-	Antitoxin
-	85506..85692	-	187	NuclAT_0	-	-
-	85506..85692	-	187	NuclAT_0	-	-
-	85506..85692	-	187	NuclAT_0	-	-
-	85506..85692	-	187	NuclAT_0	-	-
CCL28_RS26310	85704..86423	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CCL28_RS26315	86420..86854	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
CCL28_RS26320	86909..88867	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..134899	134899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77741 WP_001312861.1 NZ_CP021871:c83978-83820 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77741 NZ_CP021871:c83978-83820 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT77741 NZ_CP021871:c84062-84022 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin