Detailed information of TA system TA077692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51976..52633
• Replicon: plasmid unitig_1
• Accession: NZ_CP021853
• Organism: Proteus mirabilis strain AR_0156

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM403_RS20065
• Protein ID: WP_000270043.1
• Coordinates: 52283..52633 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM403_RS20060
• Protein ID: WP_000124640.1
• Coordinates: 51976..52278 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM403_RS20010	47601..48029	+	429	WP_000591074.1	hypothetical protein	-
AM403_RS20015	48086..48445	+	360	WP_000422768.1	hypothetical protein	-
AM403_RS20020	48445..48891	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
AM403_RS20025	48888..49406	+	519	WP_000210756.1	nitrite reductase	-
AM403_RS20030	49406..49636	+	231	WP_000972663.1	hypothetical protein	-
AM403_RS20035	49623..50480	+	858	WP_001167032.1	hypothetical protein	-
AM403_RS20045	50711..51238	+	528	WP_001236377.1	thermonuclease family protein	-
AM403_RS20050	51296..51568	+	273	WP_001043047.1	HU family DNA-binding protein	-
AM403_RS20055	51656..51949	+	294	WP_001239997.1	chromosome segregation protein ParM	-
AM403_RS20060	51976..52278	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM403_RS20065	52283..52633	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM403_RS20070	52796..53344	+	549	WP_001061574.1	transcriptional regulator	-
AM403_RS20080	53685..53879	+	195	WP_000343597.1	hypothetical protein	-
AM403_RS20085	53890..54261	+	372	WP_000516916.1	hypothetical protein	-
AM403_RS20090	54254..54724	+	471	WP_001281821.1	hypothetical protein	-
AM403_RS20095	54739..55074	-	336	WP_000683476.1	hypothetical protein	-
AM403_RS20100	55171..55659	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM403_RS20105	55662..56159	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / sul1 / blaKPC-2 / tet(D) / floR / sul2 / dfrA14 / ARR-3 / cmlA1 / blaOXA-10	-	1..180262	180262
	-	inside	Integron	-	-	1..180262	180261

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T77692 WP_000270043.1 NZ_CP021853:c52633-52283 [Proteus mirabilis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T77692 NZ_CP021853:c52633-52283 [Proteus mirabilis]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT77692 WP_000124640.1 NZ_CP021853:c52278-51976 [Proteus mirabilis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT77692 NZ_CP021853:c52278-51976 [Proteus mirabilis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9