Detailed information of TA system TA077687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5017587..5018189
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CCL06_RS25415
• Protein ID: WP_025759559.1
• Coordinates: 5017878..5018189 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F1AZU3
• Locus tag: CCL06_RS24585
• Protein ID: WP_025759558.1
• Coordinates: 5017587..5017877 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS24570	5015085..5015987	+	903	WP_025759554.1	formate dehydrogenase subunit beta	-
CCL06_RS24575	5015984..5016619	+	636	WP_025759555.1	formate dehydrogenase cytochrome b556 subunit	-
CCL06_RS24580	5016616..5017545	+	930	WP_025759557.1	formate dehydrogenase accessory protein FdhE	-
CCL06_RS24585	5017587..5017877	-	291	WP_025759558.1	helix-turn-helix domain-containing protein	Antitoxin
CCL06_RS25415	5017878..5018189	-	312	WP_025759559.1	hypothetical protein	Toxin
CCL06_RS24595	5018417..5019334	+	918	WP_025759560.1	alpha/beta hydrolase	-
CCL06_RS24600	5019346..5020287	-	942	WP_025759561.1	fatty acid biosynthesis protein FabY	-
CCL06_RS24605	5020332..5020769	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CCL06_RS24610	5020766..5021647	-	882	WP_045285692.1	virulence factor BrkB family protein	-
CCL06_RS24615	5021641..5022240	-	600	WP_025759562.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12252.27 Da
• Isoelectric Point: 9.7328

>T77687 WP_025759559.1 NZ_CP021851:c5018189-5017878 [Enterobacter cloacae]
MLFIETDIFTEDVKALLDDDEYHKLQVFLATQPDYGDVIQHTGDLRKIRWLAKGRGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSASEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T77687 NZ_CP021851:c5018189-5017878 [Enterobacter cloacae]
ATGTTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAGCGTTACTGGATGATGACGAATACCATAAACTTCAGGT
TTTCCTTGCAACGCAGCCTGATTATGGCGATGTGATCCAGCATACAGGCGATTTACGAAAAATTCGCTGGCTTGCCAAAG
GGAGAGGAAAACGCGGGGGCGTACGCGTTATCTATTTTCACCGCACGCGTGAGTTTGAAATTAGGCTGCTGCTCATCTAT
CGCAAAGGAATCAAAGATGACCTGTCAGCAAGCGAGAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10755.26 Da
• Isoelectric Point: 10.4747

>AT77687 WP_025759558.1 NZ_CP021851:c5017877-5017587 [Enterobacter cloacae]
MDKALFERLTQSMAQMNEIDEGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSQ

Nucleotide

• Length: 291 bp

>AT77687 NZ_CP021851:c5017877-5017587 [Enterobacter cloacae]
ATGGATAAGGCGCTGTTTGAGCGGTTAACCCAAAGCATGGCGCAAATGAACGAAATCGACGAGGGCAAACGAGAGCCATC
GCGCACCTTTCAAATCGATGCGATGAAAATTAAAGAAATCCGTCAGGCCTCCGGTTTATCACAGAGTAAATTCGCGAATC
TCATCTCTGTCAGTGTTGATACGTTGCGTAACTGGGAACAGGGTAGACGTTCGCCTACGGGGCCGGCTAAAGCGCTGTTA
CGTGCTATTGCCAACGATCCTCAGCATGTCATCTCTGCTCTCTCACAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F1AZU3