Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3972756..3973254 Replicon chromosome
Accession NZ_CP021851
Organism Enterobacter cloacae strain A1137

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CCL06_RS19395 Protein ID WP_080276783.1
Coordinates 3972756..3972896 (+) Length 47 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CCL06_RS19400 Protein ID WP_025759703.1
Coordinates 3972907..3973254 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCL06_RS19375 3969878..3971047 + 1170 WP_088244439.1 HlyD family efflux transporter periplasmic adaptor subunit -
CCL06_RS19380 3971140..3971466 - 327 WP_025760023.1 DUF1493 family protein -
CCL06_RS19385 3971460..3971910 - 451 Protein_3726 hypothetical protein -
CCL06_RS25400 3972235..3972387 + 153 WP_161493424.1 ash family protein -
CCL06_RS19390 3972401..3972674 + 274 Protein_3728 hypothetical protein -
CCL06_RS19395 3972756..3972896 + 141 WP_080276783.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CCL06_RS19400 3972907..3973254 + 348 WP_025759703.1 HigA family addiction module antidote protein Antitoxin
CCL06_RS19405 3973269..3974000 - 732 WP_025759704.1 helix-turn-helix transcriptional regulator -
CCL06_RS19410 3974097..3974516 + 420 WP_025759706.1 hypothetical protein -
CCL06_RS19415 3974519..3975037 - 519 WP_088244440.1 hypothetical protein -
CCL06_RS19420 3975122..3976303 - 1182 WP_025759709.1 PLP-dependent aminotransferase family protein -
CCL06_RS19425 3976324..3977211 - 888 WP_047959299.1 LysR family transcriptional regulator -
CCL06_RS19430 3977309..3977911 + 603 WP_025759711.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-40)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 47 a.a.        Molecular weight: 5545.41 Da        Isoelectric Point: 10.2413

>T77685 WP_080276783.1 NZ_CP021851:3972756-3972896 [Enterobacter cloacae]
MYEALNPPLKGYFSIRVNKQYRLVFRWTAGKADDIYLSPHKYTQHK

Download         Length: 141 bp

>T77685 NZ_CP021851:3972756-3972896 [Enterobacter cloacae]
ATGTATGAAGCATTGAATCCTCCGTTGAAAGGCTATTTTTCAATCCGGGTCAACAAACAATACAGGCTCGTATTTCGCTG
GACAGCGGGTAAAGCAGACGATATCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13025.96 Da        Isoelectric Point: 8.5279

>AT77685 WP_025759703.1 NZ_CP021851:3972907-3973254 [Enterobacter cloacae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNSISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IREAQSNSRTQEELSRIKTVAEVMAKRKSGQPDVA

Download         Length: 348 bp

>AT77685 NZ_CP021851:3972907-3973254 [Enterobacter cloacae]
ATGACACTTCAGCAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTACGAGTACCTTGAACCGCTCAATCT
TAAAATCAGCGATCTGGCGGAGATGCTTAATGTCCACCGCAATTCCATCAGCGCATTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCAATAAAACTGGCAAAAGCCTTTGATACCACTATTGAATTTTGGCTGAATTTACAACTGAACGTCGAT
ATCCGGGAAGCGCAATCTAATTCCAGGACGCAGGAGGAATTAAGCCGTATAAAGACCGTTGCAGAAGTCATGGCTAAAAG
GAAATCTGGTCAGCCGGATGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References