Detailed information of TA system TA077684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3370400..3371060
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CCL06_RS16660
• Protein ID: WP_071994086.1
• Coordinates: 3370686..3371060 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F1AN45
• Locus tag: CCL06_RS16655
• Protein ID: WP_025760080.1
• Coordinates: 3370400..3370702 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS16620	3365733..3366437	-	705	WP_161493421.1	protein phosphatase 2C domain-containing protein	-
CCL06_RS16625	3366434..3367765	-	1332	WP_072010675.1	serine/threonine protein kinase	-
CCL06_RS25315	3368220..3368837	-	618	WP_025760086.1	hypothetical protein	-
CCL06_RS16640	3368943..3369434	-	492	WP_025760084.1	Hcp family type VI secretion system effector	-
CCL06_RS16645	3369639..3370016	-	378	Protein_3204	hypothetical protein	-
CCL06_RS16650	3370014..3370226	+	213	Protein_3205	5'-nucleotidase C-terminal domain-containing protein	-
CCL06_RS16655	3370400..3370702	-	303	WP_025760080.1	XRE family transcriptional regulator	Antitoxin
CCL06_RS16660	3370686..3371060	-	375	WP_071994086.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCL06_RS16665	3371237..3372130	-	894	WP_032672695.1	LysR family transcriptional regulator	-
CCL06_RS16670	3372301..3372909	+	609	WP_025760078.1	glutathione S-transferase family protein	-
CCL06_RS16675	3372952..3373446	-	495	WP_025760076.1	flavin reductase family protein	-
CCL06_RS16680	3373433..3374758	-	1326	WP_025760075.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14424.45 Da
• Isoelectric Point: 9.2040

>T77684 WP_071994086.1 NZ_CP021851:c3371060-3370686 [Enterobacter cloacae]
VWTINTTDRFDYWFRSLDDTDRACVLAALLVLREKGPALSRPYADTLKGSRHSNMKELRIQSKCDPLRAFFAFDPNRTGI
VLCAGNKAGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Nucleotide

• Length: 375 bp

>T77684 NZ_CP021851:c3371060-3370686 [Enterobacter cloacae]
GTGTGGACAATTAACACGACGGACAGGTTTGATTACTGGTTTCGTTCACTTGACGATACTGACCGAGCATGCGTGCTGGC
AGCACTGCTTGTTTTGCGCGAAAAGGGGCCGGCATTGTCGAGACCCTATGCTGATACGCTTAAAGGCTCTCGTCACAGTA
ACATGAAAGAGTTGCGCATTCAGAGTAAGTGTGATCCGTTGCGGGCATTTTTCGCATTTGACCCAAACCGAACGGGCATT
GTGCTTTGTGCAGGCAATAAGGCCGGTAACGAAAGACGATTTTACGATGAGATGCTTCTGGTGGCTGACCGGGAATTCAC
TCACTGGTTGAATACATTAAAAGAGAGGACGTTACGTGATGGGCAGAAACCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11025.80 Da
• Isoelectric Point: 5.7251

>AT77684 WP_025760080.1 NZ_CP021851:c3370702-3370400 [Enterobacter cloacae]
MGRNLEQLIADEKPDVVTSAQAMAADILLNINLAELREKVQKTQVEMAHALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLKLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT77684 NZ_CP021851:c3370702-3370400 [Enterobacter cloacae]
ATGGGCAGAAACCTTGAGCAGCTTATAGCGGATGAAAAACCGGATGTTGTTACCAGCGCACAGGCAATGGCTGCTGACAT
TTTGCTTAACATCAACCTTGCAGAGCTACGTGAAAAGGTGCAAAAAACGCAGGTCGAAATGGCGCATGCGCTGGGTATCA
GGCAGCCCACCGTCGCGGTTATGGAAAAGCCCGGGCGCGATCTGAAACTCTCTTCACTCAAGCGCTACGTTGAGGCAGCG
GGTGGAAAACTTAAGCTTGACGTAGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F1AN45