Detailed information of TA system TA077673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1359178..1359827
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CCL06_RS06565
• Protein ID: WP_025759526.1
• Coordinates: 1359465..1359827 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F1AUZ8
• Locus tag: CCL06_RS06560
• Protein ID: WP_025759525.1
• Coordinates: 1359178..1359477 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS06550	1355547..1357520	+	1974	WP_088244104.1	PhoX family phosphatase	-
CCL06_RS06555	1357623..1359011	+	1389	WP_025759524.1	phenylalanine transporter	-
CCL06_RS06560	1359178..1359477	-	300	WP_025759525.1	helix-turn-helix transcriptional regulator	Antitoxin
CCL06_RS06565	1359465..1359827	-	363	WP_025759526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCL06_RS06570	1360031..1361269	+	1239	WP_088244105.1	MFS transporter	-
CCL06_RS06575	1361285..1362310	+	1026	WP_025759528.1	sugar phosphate isomerase/epimerase	-
CCL06_RS06580	1362303..1363445	+	1143	WP_025759529.1	Gfo/Idh/MocA family oxidoreductase	-
CCL06_RS06585	1363521..1364492	+	972	WP_045286101.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1293416..1368952	75536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14027.06 Da
• Isoelectric Point: 6.7211

>T77673 WP_025759526.1 NZ_CP021851:c1359827-1359465 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGKPIRAFFAFDPDRKAI
VLYAGDKTGCNQKRFYKEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T77673 NZ_CP021851:c1359827-1359465 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACGGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCATTGAAAGAAGATGTGCTGGC
GATGATGCATATCCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATATGTAGACACCGTAAAGGCTTCAGCCTATGCGA
ATATGAAAGAGTTACGCATTCAGCATGCGGGCAAACCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGTAAAGCGATT
GTGCTCTATGCAGGAGACAAAACCGGTTGTAACCAAAAACGGTTTTATAAAGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCTGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10903.64 Da
• Isoelectric Point: 9.8627

>AT77673 WP_025759525.1 NZ_CP021851:c1359477-1359178 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNVSQTELANAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT77673 NZ_CP021851:c1359477-1359178 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGAAGTGATGCG
CAGAATTGTTGCCCTGAATCAATTGCGGGAAGAGCTCAATGTTTCGCAGACGGAACTGGCCAACGCAATGGGGGTAAAAC
AACCCACTGTAGCGAAAATCGAACAGCCGGGTAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCCCTCGGC
GGTGAACTGAGCATTGATGTCACGTTACCGAATGGGAAACGGATAGCGTTTCAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F1AUZ8