Detailed information of TA system TA077666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31636..32366
• Replicon: chromosome
• Accession: NZ_CP021851
• Organism: Enterobacter cloacae strain A1137

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A443XYC4
• Locus tag: CCL06_RS00155
• Protein ID: WP_025756994.1
• Coordinates: 31636..31950 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCL06_RS00160
• Protein ID: WP_152001941.1
• Coordinates: 31947..32366 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCL06_RS00130	27303..28391	+	1089	WP_025756990.1	cellulase family glycosylhydrolase	-
CCL06_RS00135	28294..29478	-	1185	WP_088244421.1	multidrug efflux MFS transporter EmrD	-
CCL06_RS00140	29654..30487	-	834	WP_025756992.1	DMT family transporter	-
CCL06_RS00145	30550..30996	-	447	WP_025756993.1	GNAT family N-acetyltransferase	-
CCL06_RS00150	31061..31150	-	90	WP_020684667.1	type I toxin-antitoxin system toxin TisB	-
CCL06_RS00155	31636..31950	+	315	WP_025756994.1	type II toxin-antitoxin system HigB family toxin	Toxin
CCL06_RS00160	31947..32366	+	420	WP_152001941.1	helix-turn-helix domain-containing protein	Antitoxin
CCL06_RS00165	32478..32576	+	99	WP_071830013.1	ilvB operon leader peptide IvbL	-
CCL06_RS00170	32683..34371	+	1689	WP_025756996.1	acetolactate synthase large subunit	-
CCL06_RS00175	34375..34662	+	288	WP_006808802.1	acetolactate synthase small subunit	-
CCL06_RS00180	34744..35337	+	594	WP_025756997.1	transcriptional regulator UhpA	-
CCL06_RS00185	35334..36839	+	1506	WP_025756998.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12358.46 Da
• Isoelectric Point: 10.2219

>T77666 WP_025756994.1 NZ_CP021851:31636-31950 [Enterobacter cloacae]
MHLISMKAILDAVTHFPQHKEELLFLGRTIEKSHCATPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T77666 NZ_CP021851:31636-31950 [Enterobacter cloacae]
ATGCATTTAATCTCGATGAAAGCTATTTTGGATGCGGTAACCCACTTTCCGCAGCATAAGGAAGAACTCCTCTTTCTTGG
TCGCACCATAGAAAAGAGTCATTGCGCAACGCCTGCTGCGCTGAAGAAACTTTTTCCGACGCTGGATAATTTTAAGTATC
TGGATAAGCATTATGTTATTGATATTGCAAACAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15494.13 Da
• Isoelectric Point: 5.6761

>AT77666 WP_152001941.1 NZ_CP021851:31947-32366 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHFEANRPEIVALRKEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLSARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT77666 NZ_CP021851:31947-32366 [Enterobacter cloacae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAACTGGTTGAGTATCTGCTCATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATCAGCCATTTCGAAGCCAACCGGCCAGAGATCGTCGCATTACGCAAGGAGATGGAGTCCGTTCCTGTCGGC
ATCGCGGTTTTAAGAACCTTGATGGATCAATATAAACTGACAATATCAGATTTTCAGGACGAAATTGGCAGTAAATCAAT
GGTTTCACGGGTTTTGAATGGCCAGAGACAACTTACTCTGAACCATATTAAAAAGCTGTCGGCCAGATTTGGGGTATCAC
CTGCGCTGTTTATTGAGTAA

Structures

Toxin

• AlphaFold DB: A0A443XYC4