Detailed information of TA system TA077661

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47486..47755
• Replicon: plasmid pEC1515-3
• Accession: NZ_CP021847
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CDH89_RS27865
• Protein ID: WP_001312861.1
• Coordinates: 47597..47755 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47486..47551 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS27840	43259..43786	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CDH89_RS27845	43842..44075	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CDH89_RS27850	44134..46098	+	1965	WP_079976098.1	ParB/RepB/Spo0J family partition protein	-
CDH89_RS27855	46167..46601	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-
CDH89_RS27860	46598..47317	+	720	WP_000116352.1	plasmid SOS inhibition protein A	-
CDH89_RS28450	47329..47517	-	189	WP_001299721.1	hypothetical protein	-
-	47329..47553	+	225	NuclAT_1	-	-
-	47329..47553	+	225	NuclAT_1	-	-
-	47329..47553	+	225	NuclAT_1	-	-
-	47329..47553	+	225	NuclAT_1	-	-
-	47486..47551	+	66	-	-	Antitoxin
CDH89_RS27865	47597..47755	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CDH89_RS28645	48446..48652	+	207	WP_000547971.1	hypothetical protein	-
CDH89_RS27880	48677..48964	+	288	WP_000107535.1	hypothetical protein	-
CDH89_RS27885	49083..49904	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CDH89_RS27890	50201..50848	-	648	WP_157698281.1	transglycosylase SLT domain-containing protein	-
CDH89_RS27895	51125..51508	+	384	WP_000124979.1	relaxosome protein TraM	-
CDH89_RS27900	51699..52385	+	687	WP_000332493.1	PAS domain-containing protein	-
CDH89_RS27905	52479..52706	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / ant(3'')-Ia / lnu(F) / sul3	-	1..84091	84091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77661 WP_001312861.1 NZ_CP021847:47597-47755 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77661 NZ_CP021847:47597-47755 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT77661 NZ_CP021847:47486-47551 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin