Detailed information of TA system TA077656

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41910..42180
• Replicon: plasmid pEC1515-3
• Accession: NZ_CP021847
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CDH89_RS27825
• Protein ID: WP_001312861.1
• Coordinates: 42022..42180 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41910..41973 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS27780	36957..37211	+	255	WP_071598075.1	hypothetical protein	-
CDH89_RS27800	37684..38211	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CDH89_RS27805	38267..38500	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CDH89_RS27810	38559..40523	+	1965	WP_079976098.1	ParB/RepB/Spo0J family partition protein	-
CDH89_RS27815	40592..41026	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-
CDH89_RS27820	41023..41742	+	720	WP_000116352.1	plasmid SOS inhibition protein A	-
CDH89_RS28440	41754..41942	-	189	WP_001299721.1	hypothetical protein	-
-	41754..41978	+	225	NuclAT_0	-	-
-	41754..41978	+	225	NuclAT_0	-	-
-	41754..41978	+	225	NuclAT_0	-	-
-	41754..41978	+	225	NuclAT_0	-	-
-	41910..41973	-	64	-	-	Antitoxin
CDH89_RS27825	42022..42180	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CDH89_RS28445	42475..42786	+	312	WP_198320231.1	hypothetical protein	-
CDH89_RS27840	43259..43786	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CDH89_RS27845	43842..44075	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CDH89_RS27850	44134..46098	+	1965	WP_079976098.1	ParB/RepB/Spo0J family partition protein	-
CDH89_RS27855	46167..46601	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / ant(3'')-Ia / lnu(F) / sul3	-	1..84091	84091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77656 WP_001312861.1 NZ_CP021847:42022-42180 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77656 NZ_CP021847:42022-42180 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT77656 NZ_CP021847:c41973-41910 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin