Detailed information of TA system TA077650

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28564..28828
• Replicon: plasmid pEC1515-1
• Accession: NZ_CP021845
• Organism: Escherichia coli strain EC1515

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CDH89_RS26205
• Protein ID: WP_001387489.1
• Coordinates: 28564..28716 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28768..28828 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH89_RS26180	23830..25998	+	2169	WP_023518847.1	DotA/TraY family protein	-
CDH89_RS26185	26069..26731	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CDH89_RS26190	26803..27012	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CDH89_RS28600	27404..27580	+	177	WP_001054897.1	hypothetical protein	-
CDH89_RS26195	27645..27941	-	297	WP_011264046.1	hypothetical protein	-
CDH89_RS26200	28241..28492	+	252	WP_001291964.1	hypothetical protein	-
CDH89_RS26205	28564..28716	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	28768..28828	+	61	NuclAT_0	-	Antitoxin
-	28768..28828	+	61	NuclAT_0	-	Antitoxin
-	28768..28828	+	61	NuclAT_0	-	Antitoxin
-	28768..28828	+	61	NuclAT_0	-	Antitoxin
CDH89_RS28385	29031..29378	+	348	Protein_31	protein finQ	-
CDH89_RS26215	29564..30826	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
CDH89_RS26220	31150..32295	+	1146	WP_015056382.1	class C beta-lactamase CMY-4	-
CDH89_RS26225	32389..32922	+	534	WP_001221666.1	lipocalin family protein	-
CDH89_RS26230	32919..33236	-	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / aac(3)-IId / ant(3'')-Ia	-	1..125099	125099
	-	flank	IS/Tn	blaCMY-4	-	29564..32295	2731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T77650 WP_001387489.1 NZ_CP021845:c28716-28564 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T77650 NZ_CP021845:c28716-28564 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT77650 NZ_CP021845:28768-28828 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin