Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4057716..4058257 Replicon chromosome
Accession NZ_CP021844
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name yafQ Uniprot ID A0A246NT41
Locus tag CDH89_RS20725 Protein ID WP_000615977.1
Coordinates 4057979..4058257 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A7B3MMA0
Locus tag CDH89_RS20720 Protein ID WP_021576987.1
Coordinates 4057716..4057976 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS20705 4053782..4055875 + 2094 WP_033815253.1 flagellar biosynthesis protein FlhA -
CDH89_RS20710 4056084..4056581 - 498 WP_000006266.1 REP-associated tyrosine transposase RayT -
CDH89_RS20715 4056757..4057506 - 750 WP_000056850.1 C40 family peptidase -
CDH89_RS20720 4057716..4057976 + 261 WP_021576987.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CDH89_RS20725 4057979..4058257 + 279 WP_000615977.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CDH89_RS20730 4058413..4059153 + 741 WP_001225679.1 murein L,D-transpeptidase -
CDH89_RS20735 4059124..4059891 - 768 WP_000333380.1 class II glutamine amidotransferase -
CDH89_RS20740 4060097..4060675 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4056757..4066509 9752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10842.67 Da        Isoelectric Point: 10.0702

>T77645 WP_000615977.1 NZ_CP021844:4057979-4058257 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNILPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T77645 NZ_CP021844:4057979-4058257 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.78 Da        Isoelectric Point: 7.0129

>AT77645 WP_021576987.1 NZ_CP021844:4057716-4057976 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNKLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT77645 NZ_CP021844:4057716-4057976 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATAAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NT41

Antitoxin

Source ID Structure
AlphaFold DB A0A7B3MMA0

References