Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2002578..2003080 Replicon chromosome
Accession NZ_CP021844
Organism Escherichia coli strain EC1515

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CDH89_RS10120 Protein ID WP_000767829.1
Coordinates 2002826..2003080 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CDH89_RS10115 Protein ID WP_001259255.1
Coordinates 2002578..2002829 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH89_RS10085 1997756..1998823 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CDH89_RS10090 1998823..1999893 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
CDH89_RS10095 1999896..2001194 - 1299 WP_000009595.1 histidinol dehydrogenase -
CDH89_RS10100 2001200..2002099 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CDH89_RS10110 2002245..2002295 - 51 WP_001364200.1 his operon leader peptide -
CDH89_RS10115 2002578..2002829 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CDH89_RS10120 2002826..2003080 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CDH89_RS10125 2003163..2003987 + 825 WP_072666641.1 SDR family oxidoreductase -
CDH89_RS10130 2004033..2004962 + 930 WP_000803340.1 LysR family transcriptional regulator -
CDH89_RS28175 2005177..2005239 + 63 WP_010723108.1 membrane protein YoeI -
CDH89_RS10135 2005229..2006587 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CDH89_RS10140 2006766..2007824 + 1059 WP_000492339.1 transport protein YeeE -
CDH89_RS10145 2007838..2008065 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T77631 WP_000767829.1 NZ_CP021844:2002826-2003080 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T77631 NZ_CP021844:2002826-2003080 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT77631 WP_001259255.1 NZ_CP021844:2002578-2002829 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT77631 NZ_CP021844:2002578-2002829 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References