Detailed information of TA system TA077614

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76734..76998
• Replicon: plasmid pEC974-1
• Accession: NZ_CP021841
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CDH88_RS26635
• Protein ID: WP_001387489.1
• Coordinates: 76846..76998 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	76734..76794 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS26610	72326..72643	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
CDH88_RS26615	72640..73173	-	534	WP_001221666.1	lipocalin family protein	-
CDH88_RS26620	73267..74412	-	1146	WP_063859698.1	class C beta-lactamase CMY-111	-
CDH88_RS26625	74736..75998	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
CDH88_RS28350	76184..76531	-	348	Protein_90	protein finQ	-
-	76734..76794	-	61	NuclAT_0	-	Antitoxin
-	76734..76794	-	61	NuclAT_0	-	Antitoxin
-	76734..76794	-	61	NuclAT_0	-	Antitoxin
-	76734..76794	-	61	NuclAT_0	-	Antitoxin
CDH88_RS26635	76846..76998	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CDH88_RS26640	77070..77321	-	252	WP_001291964.1	hypothetical protein	-
CDH88_RS26645	77621..77917	+	297	WP_011264046.1	hypothetical protein	-
CDH88_RS28565	77982..78158	-	177	WP_001054897.1	hypothetical protein	-
CDH88_RS26650	78550..78759	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CDH88_RS26655	78831..79493	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CDH88_RS26660	79564..81732	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / aac(3)-IId / blaCMY-111	-	1..118934	118934
	-	flank	IS/Tn	blaCMY-111	-	73267..75998	2731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T77614 WP_001387489.1 NZ_CP021841:76846-76998 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T77614 NZ_CP021841:76846-76998 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT77614 NZ_CP021841:c76794-76734 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin