Detailed information of TA system TA077542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2438745..2439363
• Replicon: chromosome
• Accession: NZ_CP021825
• Organism: Sinorhizobium meliloti strain KH35c

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H0FWR0
• Locus tag: CDO23_RS12150
• Protein ID: WP_003527306.1
• Coordinates: 2438745..2439041 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO23_RS12155
• Protein ID: WP_010969010.1
• Coordinates: 2439055..2439363 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO23_RS12130	2434718..2435746	-	1029	WP_088217783.1	porin	-
CDO23_RS12135	2435917..2436930	-	1014	WP_164821200.1	site-specific integrase	-
CDO23_RS12140	2437027..2438064	-	1038	WP_027987990.1	porin	-
CDO23_RS12145	2438392..2438697	+	306	WP_028010116.1	hypothetical protein	-
CDO23_RS12150	2438745..2439041	+	297	WP_003527306.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO23_RS12155	2439055..2439363	+	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO23_RS12160	2439372..2439605	+	234	WP_028010117.1	hypothetical protein	-
CDO23_RS12170	2440033..2441154	+	1122	WP_028010118.1	pyridoxal phosphate-dependent aminotransferase	-
CDO23_RS12175	2441176..2441442	-	267	WP_028010119.1	sel1 repeat family protein	-
CDO23_RS12180	2442029..2442370	+	342	WP_003527314.1	DUF2147 domain-containing protein	-
CDO23_RS12190	2442694..2443395	-	702	WP_028010120.1	glutathione S-transferase N-terminal domain-containing protein	-
CDO23_RS12195	2443631..2443804	+	174	WP_003527318.1	hypothetical protein	-
CDO23_RS12200	2443818..2443991	+	174	WP_003527319.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11601.01 Da
• Isoelectric Point: 6.7262

>T77542 WP_003527306.1 NZ_CP021825:2438745-2439041 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPSDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77542 NZ_CP021825:2438745-2439041 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTCCGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77542 WP_010969010.1 NZ_CP021825:2439055-2439363 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77542 NZ_CP021825:2439055-2439363 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAACGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGATTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: H0FWR0