Detailed information of TA system TA077525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1768497..1769115
• Replicon: chromosome
• Accession: NZ_CP021822
• Organism: Sinorhizobium meliloti strain KH46

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO24_RS08400
• Protein ID: WP_010969011.1
• Coordinates: 1768819..1769115 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO24_RS08395
• Protein ID: WP_010969010.1
• Coordinates: 1768497..1768805 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO24_RS08350	1763576..1763896	-	321	WP_003527320.1	YnfA family protein	-
CDO24_RS08355	1763971..1764144	-	174	WP_010969006.1	hypothetical protein	-
CDO24_RS08360	1764158..1764331	-	174	WP_003527318.1	hypothetical protein	-
CDO24_RS08365	1764567..1765268	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
CDO24_RS08370	1765492..1765833	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
CDO24_RS08375	1766418..1766684	+	267	WP_003527312.1	sel1 repeat family protein	-
CDO24_RS08380	1766706..1767827	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
CDO24_RS08390	1768256..1768486	-	231	WP_026029805.1	hypothetical protein	-
CDO24_RS08395	1768497..1768805	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO24_RS08400	1768819..1769115	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO24_RS08405	1769163..1769468	-	306	WP_003527305.1	hypothetical protein	-
CDO24_RS08410	1769796..1770833	+	1038	WP_003527304.1	porin	-
CDO24_RS08415	1770943..1772010	-	1068	WP_014526650.1	site-specific integrase	-
CDO24_RS08420	1772051..1773100	-	1050	WP_010969013.1	porin	-
CDO24_RS08425	1773314..1773802	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77525 WP_010969011.1 NZ_CP021822:c1769115-1768819 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77525 NZ_CP021822:c1769115-1768819 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77525 WP_010969010.1 NZ_CP021822:c1768805-1768497 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77525 NZ_CP021822:c1768805-1768497 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61