Detailed information of TA system TA077494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1959253..1959871
• Replicon: chromosome
• Accession: NZ_CP021812
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO26_RS10480
• Protein ID: WP_010969011.1
• Coordinates: 1959253..1959549 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO26_RS10485
• Protein ID: WP_010969010.1
• Coordinates: 1959563..1959871 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS10455 (CDO26_10465)	1954566..1955054	+	489	WP_013844230.1	BA14K family protein	-
CDO26_RS10460 (CDO26_10470)	1955269..1956318	+	1050	WP_013844229.1	porin	-
CDO26_RS10465 (CDO26_10475)	1956359..1957425	+	1067	Protein_1992	site-specific integrase	-
CDO26_RS10470 (CDO26_10480)	1957535..1958572	-	1038	WP_003527304.1	porin	-
CDO26_RS10475 (CDO26_10485)	1958900..1959205	+	306	WP_003527305.1	hypothetical protein	-
CDO26_RS10480 (CDO26_10490)	1959253..1959549	+	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO26_RS10485 (CDO26_10495)	1959563..1959871	+	309	WP_010969010.1	HigA family addiction module antitoxin	Antitoxin
CDO26_RS10490 (CDO26_10500)	1959861..1960112	+	252	WP_042708639.1	hypothetical protein	-
CDO26_RS10500 (CDO26_10510)	1960451..1960780	-	330	WP_280640935.1	hypothetical protein	-
CDO26_RS39685	1960954..1961035	+	82	Protein_1999	nitrogen fixation protein FixA	-
CDO26_RS10505 (CDO26_10515)	1961197..1962321	+	1125	Protein_2000	TlpA disulfide reductase family protein	-
CDO26_RS39690 (CDO26_10520)	1963001..1963201	-	201	WP_088197207.1	hypothetical protein	-
CDO26_RS10515 (CDO26_10525)	1963253..1963618	-	366	WP_014529432.1	hypothetical protein	-
CDO26_RS10520 (CDO26_10530)	1963673..1963915	-	243	WP_028011801.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77494 WP_010969011.1 NZ_CP021812:1959253-1959549 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77494 NZ_CP021812:1959253-1959549 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77494 WP_010969010.1 NZ_CP021812:1959563-1959871 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77494 NZ_CP021812:1959563-1959871 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61