Detailed information of TA system TA077463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1010043..1010661
• Replicon: chromosome
• Accession: NZ_CP021804
• Organism: Sinorhizobium meliloti strain T073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO28_RS05250
• Protein ID: WP_010969011.1
• Coordinates: 1010043..1010339 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO28_RS05255
• Protein ID: WP_010969010.1
• Coordinates: 1010353..1010661 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO28_RS05225	1005356..1005844	+	489	WP_013844230.1	BA14K family protein	-
CDO28_RS05230	1006058..1007107	+	1050	WP_010969013.1	porin	-
CDO28_RS05235	1007148..1008215	+	1068	WP_014526650.1	site-specific integrase	-
CDO28_RS05240	1008325..1009362	-	1038	WP_003527304.1	porin	-
CDO28_RS05245	1009690..1009995	+	306	WP_003527305.1	hypothetical protein	-
CDO28_RS05250	1010043..1010339	+	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO28_RS05255	1010353..1010661	+	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO28_RS05260	1010672..1010902	+	231	WP_026029805.1	hypothetical protein	-
CDO28_RS05270	1011331..1012452	+	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
CDO28_RS05275	1012474..1012740	-	267	WP_003527312.1	sel1 repeat family protein	-
CDO28_RS05280	1013325..1013666	+	342	WP_010969008.1	DUF2147 domain-containing protein	-
CDO28_RS05285	1013890..1014591	-	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
CDO28_RS05290	1014827..1015000	+	174	WP_003527318.1	hypothetical protein	-
CDO28_RS05295	1015014..1015187	+	174	WP_010969006.1	hypothetical protein	-
CDO28_RS05300	1015262..1015582	+	321	WP_003527320.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77463 WP_010969011.1 NZ_CP021804:1010043-1010339 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77463 NZ_CP021804:1010043-1010339 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77463 WP_010969010.1 NZ_CP021804:1010353-1010661 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77463 NZ_CP021804:1010353-1010661 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61