Detailed information of TA system TA077448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1374932..1375550
• Replicon: chromosome
• Accession: NZ_CP021800
• Organism: Sinorhizobium meliloti strain USDA1021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO29_RS07000
• Protein ID: WP_010969011.1
• Coordinates: 1374932..1375228 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO29_RS07005
• Protein ID: WP_010969010.1
• Coordinates: 1375242..1375550 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO29_RS06975	1370245..1370733	+	489	WP_013844230.1	BA14K family protein	-
CDO29_RS06980	1370947..1371996	+	1050	WP_013844229.1	porin	-
CDO29_RS06985	1372037..1373104	+	1068	WP_017265823.1	site-specific integrase	-
CDO29_RS06990	1373214..1374251	-	1038	WP_003527304.1	porin	-
CDO29_RS06995	1374579..1374884	+	306	WP_088203194.1	hypothetical protein	-
CDO29_RS07000	1374932..1375228	+	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO29_RS07005	1375242..1375550	+	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO29_RS07010	1375540..1375791	+	252	WP_042708639.1	hypothetical protein	-
CDO29_RS07020	1376130..1377104	-	975	WP_164832917.1	non-homologous end-joining DNA ligase	-
CDO29_RS36925	1377208..1377378	+	171	WP_088203196.1	hypothetical protein	-
CDO29_RS07030	1377371..1377739	+	369	WP_088203197.1	hypothetical protein	-
CDO29_RS07035	1378274..1378639	-	366	WP_014526862.1	hypothetical protein	-
CDO29_RS07040	1378698..1378940	-	243	WP_088203198.1	hypothetical protein	-
CDO29_RS07050	1379061..1380029	-	969	WP_088203199.1	chitinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77448 WP_010969011.1 NZ_CP021800:1374932-1375228 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77448 NZ_CP021800:1374932-1375228 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77448 WP_010969010.1 NZ_CP021800:1375242-1375550 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77448 NZ_CP021800:1375242-1375550 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61