Detailed information of TA system TA077432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 512477..513095
• Replicon: chromosome
• Accession: NZ_CP021797
• Organism: Sinorhizobium meliloti strain USDA1106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO30_RS02590
• Protein ID: WP_010969011.1
• Coordinates: 512477..512773 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO30_RS02595
• Protein ID: WP_010969010.1
• Coordinates: 512787..513095 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO30_RS02575	509582..510649	+	1068	WP_014526650.1	site-specific integrase	-
CDO30_RS02580	510759..511796	-	1038	WP_003527304.1	porin	-
CDO30_RS02585	512124..512429	+	306	WP_003527305.1	hypothetical protein	-
CDO30_RS02590	512477..512773	+	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO30_RS02595	512787..513095	+	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO30_RS02600	513106..513336	+	231	WP_026029805.1	hypothetical protein	-
CDO30_RS02610	513765..514886	+	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
CDO30_RS02615	514908..515174	-	267	WP_003527312.1	sel1 repeat family protein	-
CDO30_RS02620	515759..516100	+	342	WP_010969008.1	DUF2147 domain-containing protein	-
CDO30_RS02625	516324..517025	-	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
CDO30_RS02630	517261..517434	+	174	WP_003527318.1	hypothetical protein	-
CDO30_RS02635	517448..517621	+	174	WP_010969006.1	hypothetical protein	-
CDO30_RS02640	517696..518016	+	321	WP_003527320.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77432 WP_010969011.1 NZ_CP021797:512477-512773 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77432 NZ_CP021797:512477-512773 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77432 WP_010969010.1 NZ_CP021797:512787-513095 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77432 NZ_CP021797:512787-513095 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61