Detailed information of TA system TA077417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 732827..733445
• Replicon: chromosome
• Accession: NZ_CP021793
• Organism: Sinorhizobium meliloti strain USDA1157

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO31_RS03645
• Protein ID: WP_010969011.1
• Coordinates: 733149..733445 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO31_RS03640
• Protein ID: WP_010969010.1
• Coordinates: 732827..733135 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO31_RS03595	727906..728226	-	321	WP_003527320.1	YnfA family protein	-
CDO31_RS03600	728301..728474	-	174	WP_010969006.1	hypothetical protein	-
CDO31_RS03605	728488..728661	-	174	WP_003527318.1	hypothetical protein	-
CDO31_RS03610	728897..729598	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
CDO31_RS03615	729822..730163	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
CDO31_RS03620	730748..731014	+	267	WP_003527312.1	sel1 repeat family protein	-
CDO31_RS03625	731036..732157	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
CDO31_RS03635	732586..732837	-	252	WP_198389753.1	transposase	-
CDO31_RS03640	732827..733135	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO31_RS03645	733149..733445	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO31_RS03650	733493..733798	-	306	WP_003527305.1	hypothetical protein	-
CDO31_RS03655	734126..735163	+	1038	WP_003527304.1	porin	-
CDO31_RS03660	735273..736340	-	1068	WP_014526650.1	site-specific integrase	-
CDO31_RS03665	736381..737430	-	1050	WP_010969013.1	porin	-
CDO31_RS03670	737644..738132	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77417 WP_010969011.1 NZ_CP021793:c733445-733149 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77417 NZ_CP021793:c733445-733149 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77417 WP_010969010.1 NZ_CP021793:c733135-732827 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77417 NZ_CP021793:c733135-732827 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61