Detailed information of TA system TA077383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6201880..6202475
• Replicon: chromosome
• Accession: NZ_CP021775
• Organism: Pseudomonas aeruginosa strain Pa58

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CDL16_RS30235
• Protein ID: WP_003113526.1
• Coordinates: 6202197..6202475 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDL16_RS30230
• Protein ID: WP_003133769.1
• Coordinates: 6201880..6202185 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDL16_RS30200	6196903..6197193	-	291	WP_031757220.1	DUF5447 family protein	-
CDL16_RS30205	6197369..6197641	-	273	WP_004352675.1	hypothetical protein	-
CDL16_RS30215	6198081..6198416	+	336	WP_023094905.1	hypothetical protein	-
CDL16_RS35455	6198510..6199100	+	591	WP_071536478.1	SLATT domain-containing protein	-
CDL16_RS30220	6199097..6200362	+	1266	WP_023094906.1	nucleotidyltransferase	-
CDL16_RS30225	6200446..6201438	-	993	WP_003133766.1	hypothetical protein	-
CDL16_RS30230	6201880..6202185	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
CDL16_RS30235	6202197..6202475	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDL16_RS30245	6202804..6205032	+	2229	WP_003133778.1	TonB-dependent receptor	-
CDL16_RS30250	6205103..6205750	-	648	WP_003095021.1	carbonate dehydratase	-
CDL16_RS30255	6205812..6207050	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T77383 WP_003113526.1 NZ_CP021775:c6202475-6202197 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T77383 NZ_CP021775:c6202475-6202197 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT77383 WP_003133769.1 NZ_CP021775:c6202185-6201880 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT77383 NZ_CP021775:c6202185-6201880 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3