Detailed information of TA system TA077378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5035749..5036358
• Replicon: chromosome
• Accession: NZ_CP021775
• Organism: Pseudomonas aeruginosa strain Pa58

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CDL16_RS24430
• Protein ID: WP_087921000.1
• Coordinates: 5036047..5036358 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDL16_RS24425
• Protein ID: WP_198345412.1
• Coordinates: 5035749..5035967 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDL16_RS24395	5031970..5032470	-	501	WP_000376623.1	GNAT family N-acetyltransferase	-
CDL16_RS24405	5032598..5033437	-	840	WP_000259032.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
CDL16_RS24410	5033431..5033778	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
CDL16_RS24415	5033942..5034733	-	792	WP_001206317.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
CDL16_RS24420	5034770..5035597	-	828	WP_001007673.1	oxacillin-hydrolyzing class D beta-lactamase OXA-2	-
CDL16_RS24425	5035749..5035967	-	219	WP_198345412.1	helix-turn-helix domain-containing protein	Antitoxin
CDL16_RS24430	5036047..5036358	-	312	WP_087921000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDL16_RS24435	5036547..5037005	-	459	WP_013263788.1	aminoglycoside N-acetyltransferase AAC(6')-Il	-
CDL16_RS24440	5037064..5037597	-	534	WP_000381803.1	aminoglycoside nucleotidyltransferase ANT(2'')-Ia	-
CDL16_RS24445	5037743..5038756	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
CDL16_RS35390	5038959..5039309	+	351	WP_000454193.1	DUF3330 domain-containing protein	-
CDL16_RS24455	5039435..5039995	+	561	WP_000147567.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	blaGES-1 / sul1 / ant(3'')-Ia / blaOXA-2	-	4994759..5059984	65225
	-	inside	IScluster/Tn	sul1 / ant(3'')-Ia / blaOXA-2	-	5026950..5045632	18682
	-	inside	Integron	-	-	5033881..5038756	4875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (44-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-50)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11471.11 Da
• Isoelectric Point: 9.4580

>T77378 WP_087921000.1 NZ_CP021775:c5036358-5036047 [Pseudomonas aeruginosa]
MLTVVETLLFQRQWPLYWSEEERGAFAAYIADHPAAGDVVPESGGIRKVRWSRAGSGKSGGVRVIYFTRTSEGEVVLLTL
YAKAKTDNITGAKLKEIRRALEE

Nucleotide

• Length: 312 bp

>T77378 NZ_CP021775:c5036358-5036047 [Pseudomonas aeruginosa]
ATGCTAACTGTCGTCGAAACGCTTCTCTTCCAGCGGCAATGGCCGCTTTACTGGTCGGAAGAGGAAAGAGGCGCTTTCGC
CGCCTACATCGCAGATCACCCAGCAGCCGGAGATGTTGTGCCCGAGTCCGGCGGCATCCGAAAGGTGCGTTGGTCTCGCG
CTGGATCGGGCAAGTCAGGTGGCGTACGGGTGATCTATTTCACCCGCACGTCTGAAGGTGAAGTTGTCCTGTTGACGCTT
TATGCCAAAGCAAAAACAGACAACATCACAGGTGCCAAGTTGAAGGAGATTCGTCGTGCCCTCGAAGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7484.76 Da
• Isoelectric Point: 12.1585

>AT77378 WP_198345412.1 NZ_CP021775:c5035967-5035749 [Pseudomonas aeruginosa]
MKAGQLHVVSSPVIEARKKTGLSQSQFAALMGVSVRTLQGWEQGRKQPSGAARTLLAIASTNPKAVLAVAGK

Nucleotide

• Length: 219 bp

>AT77378 NZ_CP021775:c5035967-5035749 [Pseudomonas aeruginosa]
ATGAAGGCGGGGCAGTTGCACGTCGTTTCGTCACCAGTCATCGAAGCGCGCAAAAAAACTGGCCTGTCGCAATCGCAATT
TGCCGCGCTCATGGGTGTTTCTGTGCGTACTTTGCAAGGTTGGGAGCAAGGGCGTAAGCAACCCAGCGGCGCCGCACGCA
CTTTGCTTGCAATCGCCAGCACAAATCCTAAAGCAGTGCTCGCTGTCGCAGGCAAATAA