Detailed information of TA system TA077291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4695988..4696648
• Replicon: chromosome
• Accession: NZ_CP021749
• Organism: Enterobacter cloacae complex sp. strain AR_0163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM409_RS23110
• Protein ID: WP_045910844.1
• Coordinates: 4695988..4696341 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: AM409_RS23115
• Protein ID: WP_017383313.1
• Coordinates: 4696346..4696648 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM409_RS23090 (AM409_22970)	4692419..4693342	+	924	WP_045910845.1	L,D-transpeptidase	-
AM409_RS23100 (AM409_22980)	4693833..4694750	+	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
AM409_RS23105 (AM409_22985)	4694847..4695797	+	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
AM409_RS23110 (AM409_22990)	4695988..4696341	+	354	WP_045910844.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM409_RS23115 (AM409_22995)	4696346..4696648	+	303	WP_017383313.1	XRE family transcriptional regulator	Antitoxin
AM409_RS23125 (AM409_23005)	4696937..4699126	-	2190	WP_023324924.1	TonB-dependent siderophore receptor	-
AM409_RS23130 (AM409_23010)	4699153..4700478	-	1326	WP_045910816.1	SidA/IucD/PvdA family monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13557.40 Da
• Isoelectric Point: 9.9193

>T77291 WP_045910844.1 NZ_CP021749:4695988-4696341 [Enterobacter cloacae complex sp.]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKFGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T77291 NZ_CP021749:4695988-4696341 [Enterobacter cloacae complex sp.]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTATTGCGGGAAAAGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGTTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT77291 WP_017383313.1 NZ_CP021749:4696346-4696648 [Enterobacter cloacae complex sp.]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT77291 NZ_CP021749:4696346-4696648 [Enterobacter cloacae complex sp.]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTTATGGAAAAGGCAGGTCGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTGCCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7